【摘要】 目的:研究乳腺癌BCML-TA299生物学特性、DNA状况、相关基因蛋白的表达水平及其意义。方法:应用同系小鼠原位移植BCML-TA299细胞增殖动力学组织形态学、超微结构观察、流式细胞仪以及免疫组织化学染色技术检测TA2系小鼠乳腺癌移植瘤生物异质性、基因DNA状况和蛋白质的表达水平,研究其与细胞生物特性的关系。结果:用TA2系小鼠自发乳腺癌伴肺转移组织在同系小鼠乳腺原位建立了乳腺癌肺转移A型BCML-TA299模型。历时2年多,鼠间传至第47代,移植成功率为100%。未见自发消退,肿瘤生长特性稳定。鼠间荷瘤存活中位数为51天,平均为58.8±10.03天。肿瘤倍增时间为3.11天(指数生长期)。各代移植瘤病理形态学、超微结构观察证实保持了原代瘤的特征,转移瘤与原发瘤结构一致。染色体检查为鼠类肿瘤染色体,DNA含量经流式细胞仪检测显示为异倍体肿瘤。鼠间传代移植瘤与转移瘤保持p53、cerbB-2、nm23基因异常表达。经抗癌药物敏感实验,表明对CAP(CTX、ADM、DDP)联合化疗最为敏感,而单药紫杉醇作用差。结论:本模型可为研究乳腺癌生物异质性提供有价值的实验工具。更多还原

【Abstract】 Objective:To study BCML-TA299biological characteristics DNA status and pro tein expression of lung metastasis models of breast cancer in TA2mice.Methods:using histo-logical and molecularbiological techniques such as immunocytochemical,electromicroscopic and flow cytometer methods,the implanted tumor biological characteristics,gene DNA status and pro-tein expression levels in BCML-TA299inbred mice were detected respectively.Results:A trans-plantable lung metastasis A(BCML-TA299)models of breast cancer was established on TA2inbred mice and passed47generations by successive transplantation over2years.The transplantaions successful rates were100%and no spontaneous regression of tumor ever observed.The average survival of the tumor bearing mice was58.8±10.03days.The tumor doubling times was3.11days.Histological and electro microscopic observation were identical the same characteristics as these of implant tumor and breast tumor on chromosome gene DNA status and flow cytometer studying.Drug screening tests showed that CTX ADM and DDP were most effective against BCML-TA299.Conclusion:Using transplant tumor models of BCML-TA299,studying their bio-logical characteristics were very im portant.更多还原