【摘要】 目的:探讨胃癌及癌前病变DNA检测对胃癌早期诊断的临床意义。方法:应用流式细胞术(FCM)检测了240例胃癌、癌前病变及对照组的胃粘膜细胞DNA含量(DI)、倍体和细胞增殖活性(PI)。结果:DI在重度肠上皮化生、Ⅲ级不典型增生及胃癌显著增加;重度肠上皮化生20%出现异倍体,Ⅲ级不典型增生及胃癌中分别有52.17%与71.42%出现异倍体;慢性萎缩性胃炎、重度肠上皮化生、不典型增生及胃癌PI显著增高。81例癌前病变者随访2年,15例病理诊断证实癌变,其中11例为DNA异倍体,4例为DNA二倍体,PI值也相应较高。结论:DNA检测对胃癌早期诊断是很有价值的临床生物学指标,有助于胃癌的早期诊断。更多还原

【Abstract】 Objective:To investigate t he clini cal significance of detection of DNA in gas-tric carcinoma and precancerosis o n early diagnosis of gastric carcinoma.Methods:DNA Index(DI),rate of DNA he teroploid and Proliferation Index(PI)of DNA of gastric mucosa cells were detec ted in240patients of gastric carcinoma,precancerosis,and control group by f low cytometry.Results:DI were significantly high in the patients of severe in testinal metaplasia,gradeⅢdysplas-tic gastric mucosa,and gastric carcinoma. The rate of DNA heteroploid were20%in the patients of severe intestinal metap lasia,52.17%and71.42%in gradeⅢdysplastic gastric mucosa,and gastr ic carcinoma.PI was significantly high in the patients of chronic atrophic gas tritis,severe intestinal metaplasia,gradeⅢdysplastic gastric mucosa,and gast ric carcinoma.81patients of precancerosis were followed up two years.Among 15patients of cancerization confirmed by patho-logical diagnosis11patients had DNA heteroploid.4patients had DNA diploid.PI was corre-spondingly high ,too.Conclusion:Detection of DNA is a significant clinical biological techni que on early diagnosis of gastric carcinoma and contributed to early diagnosis of gastric carcinoma.更多还原