【摘要】 目的 :建立高效液相色谱法测定人血浆中异丁司特浓度的方法。方法 :采用YWG C18色谱柱 ,以甲醇 0 .0 5mol·L-1磷酸二氢钾 (6 5∶35 ,用磷酸调pH值 3.5 )为流动相 ,检测波长 2 2 5nm ,流速 1.0mL·min-1,进样量 5 0 μL ,内标物为桂利嗪。 结果 :异丁司特标准曲线在 2～ 12 0 μg·L-1范围内线性关系良好 (r =0 .995 6 ) ,最低检测浓度为 2 μg·L-1,平均回收率为10 0 .2 % ,RSD为 4 .7% (n =5 )。结论 :该法操作简便 ,灵敏 ,准确度高 ,适用于异丁司特血药浓度的测定和药动学的研究。更多还原

【Abstract】 OBJECTIVE To establish an HPLC method for determination of ibudilast in human plasma.METHODS Chromatographic conditions included YWG-C 18 column, methanol- 0.05 mol·L -1 KH₂PO₄（65:35, pH was adjusted to 3.5 with H₃PO₄） as mobile phase, the UV detection wavelength was 225 nm, the flow rate was 1.0 mL·min -1 and cinnarizine was used as the internal standard.RESULTS The standard curve of ibudilast showed good linearity in the concentration range of 2～120 μg·L -1 （ r = 0.9956 ）, the limit of detection was 2 μg·L -1 , the average recovery was 100.2% with RSD of 4.7% （ n =5）.CONCLUSIONS The method was found to be simple, sensitive and accurate, it could be used for determining the concentration of ibudilast in human plasma for the research of clinical pharmacokinetics.更多还原