【摘要】 目的　测定丹参中丹酚酸B ,丹参素和原儿茶醛等 3种水溶性有效成分的含量。方法　采用高效液相色谱法测定。以甲醇 5 %冰醋酸为流动相进行梯度洗脱 ,以对羟基苯甲酸为内标 ,检测波长为 2 81nm ,柱温 30℃。结果　丹参素、原儿茶醛和丹酚酸B浓度分别在 3.95～ 4 7.4 μg·mL-1(r =0 .9996 ) ,5 .14～ 6 1.6 8μg·mL-1(r =0 .9999) ,16 .8～ 117.6 μg·mL-1(r =0 .9994 )范围内呈良好线性关系 ,丹参素、原儿茶醛和丹酚酸B的平均回收率分别为 10 1.4 3% (RSD =2 .88% ,n =6 ) ,99 .89% (RSD =3.0 4 % ,n =6 )和 10 3.4 3% (RSD =1.5 2 % ,n =6 )。结论　方法简便 ,分离效果好 ,能同时测定丹参中丹酚酸B、丹参素、原儿茶醛等 3种水溶性有效成分的含量 ,结果准确可靠更多还原

【Abstract】 OBJECTIVETo investigate the determination of th re e water soluble components in Salvia miltiorrhiza.METHODS The analysis was carried on a column of Hypersil C 18 (250 mm×4.6 mm,5 μm) with a mobile phase of methanol-5% acetic acid (gradient elution from 10∶ 90 to 35∶65 in 20 minutes),using 4-hydroxy-benzoic acid as the internal stand ard.The detection wavelength was at 281 nm.RESULTSThe lin earity was obtained over 3.95～47.4 μg·mL -1 (r=0.999 6)for Danshensu, 5.14～61.68 μg·mL -1 (r=0.999 9)for protocatechuic aldrhyde and 16.8～ 117.6 μg·mL -1 (r=0.999 4) for salviamolic acid B,respectively.The ave rage recoveries were 101.43%(RSD=2.88%,n=6) for Danshensu,99.89%(RSD=3.04% ,n=6)for protocatechuic aldrhyde and 103.43% (RSD=1.52%,n=6) for salvi amolic acid B,respectively.CONCLUSIONThis method appeared t o be rapid,simple and accurate.更多还原