【摘要】 目的:探讨肿瘤抑制基因PTEN和细胞凋亡关键酶Caspase-3与胃原发恶性淋巴瘤病理生物学行为的关系及其分子机制。方法:采用SABC免疫组织化学方法检测56例胃原发恶性淋巴瘤组织中PTEN基因编码产物和Caspase-3表达情况,并与胃淋巴瘤病理生物学特征进行比较分析。结果:56例胃原发恶性淋巴瘤中28例见PTEN表达,阳性率50.0％,显著低于非瘤胃粘膜阳性表达率(96.4％)(P<0.001)。肿瘤浸润深度达浆膜或穿透浆膜者PTEN表达(37.9％)显著低于未穿透肌层者(63.0％)(P<0.05);伴淋巴结转移之肿瘤PTEN表达(28.1％),显著低于不伴转移者(79.2％)(P<0.05)。Caspase-3在胃原发恶性淋巴瘤组织中阳性率76.8％,显著低于非瘤胃粘膜表层上皮阳性率96.4％(P<0.05),但高于胃固有腺体中的阳性表达率(35.7％),P<0.05。PTEN基因编码产物蛋白表达与Caspase-3的表达呈正相关(P<0.005)。结论:抑癌基因PTEN编码蛋白和Caspase-3表达在胃原发恶性淋巴瘤组织中表达下调,PTEN基因编码产物可能通过调节Caspase-3表达而影响胃淋巴瘤细胞的凋亡水平和肿瘤的病理生物学行为。PTEN和Caspase-3在胃原发恶性淋巴瘤发展过程中的作用及具体机制尚需深入探讨。更多还原

【Abstract】 Objective: Our aims were to investigate the expression of a new tumor suppressor gene PTEN and Caspase-3 in primary malignant lymphoma of stomach, and to explore the molecular mechanism of tumorigenesis and progression of primary malignant lymphoma. Methods: Fifty-six cases of primary gastric malignant lymphoma were studied by using SABC immunohistochemistry method with antibodies against PTEN protein, Caspase-3, and CD20. Results:The positive rate of PTEN in the primary gastric lymphomas was significantly lower than that in the normal gastric mucosa (50% vs 96. 4% ) ( P < 0. 005 ). The expression of PTEN in gastric lymphoma was correlated with its invasion and lymph node metastasis ( P < 0.05). The positive rate of Caspase-3 in primary malignant gastric lymphoma (76.8%) was significantly lowerthan that in the superficial gastric mucosa (96. 4% ) ( P < 0. 05 ) , while higher than that in the normal gastric glands (35.7% , P < 0.005). Additionally, the PTEN expression was positively correlated with Caspase-3 expression in primary gastric lymphoma ( P < 0.005). Conclusion: Down-regulated expression of PTEN-encoding protein and Caspase-3 played an important role in the tumorigenesis and progression of primary gastric malignant lymphoma. PTEN-encoding protein had effects on cell apoptosis of gastric lymphoma and contributed to biological behaviors of gastric lymphoma by regulating the expression of Caspase-3.更多还原