【摘要】 本文建立了卷烟烟气中的气相自由基和固相自由基的检测方法,系统地研究了卷烟烟气气相和固相自由基的测定方法和降低卷烟烟气中自由基含量的工艺和技术,系统地进行了低自由基卷烟及其对照样品的生物医学评价工作。研究结果表明:以PBN为自旋捕捉剂捕集气相自由基用ESR检测的方法和以剑桥滤片捕集固相自由基用ESR检测的方法来分别检测气相自由基和固相自由基的方法是可行的。对研制的活性碳复合滤嘴卷烟烟气自由基进行了测定,结果表明:同对照卷烟相比,试验卷烟烟气气相自由基清除率最高时可达69.1%;优化了自由基清除剂配方,确定了在增塑剂中加入1.50％的SRM溶液制成醋纤活性碳复合滤嘴再制成卷烟的工艺方法生产低自由基低焦油5mg中南海卷烟,其烟气气相自由基清除率一般可稳定达到41.2%。系统地进行了低自由基卷烟及其对照样品的生物医学评价工作,包括急性中毒试验、生殖毒性试验、免疫功能试验、致突变性试验等,所检测的各项功能性指标均表明,低自由基卷烟的毒性明显低于同品牌的普通卷烟。更多还原

【Abstract】 The determination methods for the gas-phase free radicals and solid-phase free radicals in cigarette smoke were systematically studied. A new technology to eliminate free radicals in cigarette smoke was also developed. A low-free-radical cigarette and its comparative sample cigarette were evaluated systematically by biomedical tests. PBN was finally selected to trap the gas-phase free radicals and the formed adduct was detected with ESR in the determination of gas-phase free radicals in cigarette smoke. In the technology for the elimination of free radicals in cigarette smoke, a new free radical eliminator, SRM was used. The gas-phase free radicals in the smoke of the cigarettes made with the above technology can be eliminated up to 69. 1%. The gas-phase free radical elimination efficiency of 5mg low-free-radical Zhongnanhai was up to 41. 2%. Biomedical experiments such as acute toxicity, chronic toxicity, immune function, mutation test and reproductive toxicity for this low-free-radical experiments and its comparative samples were shown that the toxicity of the low-free-radical cigarettes were considerably lower than that of the contral ones.更多还原