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GPV野毒株的分离及PCR检测方法的应用
Isolation of GPV wild strain and detection by PCR
【摘要】 在本实验中 ,用根据CPVHl标准毒核苷酸VP1_VP3和VP3两个区段基因序列设计的 2对特异性引物GRPl/GRP2和GFl/GF2 ,用该 2对引物对从鹅细小病毒野毒感染的病鹅分离的GPV野毒株进行PCR检测 ,结果 2对引物GRPl/GRP2和GFl/GR2 ,均能特异性地扩增出与预期片段大小相符的 0 .6bp和 1.6bp两个片段。回归试验的结果表明 ,该GFV野毒株的感染潜伏期为 8d ,病程为 1～ 3d ,致死率达 10 0 %。
【Abstract】 In this assay,two pairs of primers were designed according with DNA sequence from GPV Hl standard strain.Using the two pairs of primers GRPl/GRP2 and GFl/GR2 detecting the GPV wild strain isolated from infected gooses by PCR.The result showed that both of the two pairs of primers GRPl/GRP2 and GFl/GR2 could specifically amplified expected two bounds of 0.6 bp and 1.6 bp.The results of regressive assays showed that latent period was 8 d,lasting 1～3 d after infected,lethal rate was 100 % of GPV wild strain infected.
【Key words】 Goose parvovrus； wild strain； polymerase chain reaction(PCR) detection；
- 【文献出处】 中国预防兽医学报 ,Chinese Journal of Preventive Veterinary Medicine , 编辑部邮箱 ,2003年06期
- 【分类号】S852.65
- 【被引频次】20
- 【下载频次】115