【摘要】 应用MA₁0 4细胞静置培养 ,胰酶处里样本的方法从仔猪和羔羊泻便中分离到两株轮状病毒 （暂定名为PRV与SWV）。细胞培养证实所分离毒株均适应于MA_半连 10 4细胞 ,并且产生明显的CPE ;SPAIEM检查证实 ,PRV和SRV均呈现十分典型的轮状病毒的形态特征 ;理化及生物学特性检查结果显示 :PRV和SRV对有机溶剂 （乙醚、氯仿 ）、温度 （5 0℃ 30min）、酸碱度 （pH3.0 ）均有抵抗力 ;中和试验证实所分离毒株与NCDV有血清学相关性 ;RNA_PAGE有 11条RNA带 ,其带型与有关资料报道相吻合 ,为 4、2、3、2带型 ,但两株分离毒株的带型有一定的差异。更多还原

【Abstract】 Two strains of rotavirus (named as PRV and SRV) were isolated from excrement of piglet and lamb through fostering staticly MA-104 cells and dealing samples with pancreation.We verified that the two strains adapted to live in MA-104 cells and that they can all give rise to obvious cytopathogenic effect(CPE),and,PRV and SRV have just typical form and chatacteristic of rotavirus whic h was verified by SPAI and EM.The outcome of physics,chemistry and biology manifected that PRV and SRV had resistance to organic solvent (ether,chloroform),temperature (50 ℃,30 min)and acidity(pH3.0).The experiment of neutralization manifested that isolated strains had serological interrelation with NCDV.There are eleven strips in RNA-PAGE and the form of strips was 4、2、3、2,which concided with related data.But,there was little difference between the two strains in the form of strips.更多还原