【摘要】 应用伪狂犬病病毒囊膜蛋白与QuilA结合制备囊膜蛋白免疫刺激复合物 (PRV_ISCOMs) ,并通过ELISA、血清中和试验和淋巴细胞转化试验 (Brdu_ELISA法 )测定其诱导小鼠体液和细胞免疫应答水平。结果如下 :1)小鼠分别接种3、7、10ug的PRV_ISCOMs后 ,于接种后PI 7d血清中可测出ELISAIgG抗体 ,随后抗体水平逐渐升高。间隔 2 1d加强免疫后 ,ELISAIgG抗体水平进一步提高 ,且中和抗体效价均在 1∶2 2以上 ,而未加佐剂的囊膜蛋白对照组均无中和抗体 ;2 )淋巴细胞转化试验初步结果表明PRV_ISCOMs能诱导小鼠的细胞免疫应答。 3)免疫保护力测定显示免疫鼠能抵抗强毒攻击。上述结果表明PRV_ISCOMs具有良好的免疫原性 ,能诱导小鼠的体液免疫和细胞免疫应答。更多还原

【Abstract】 The PRV immunostimulating complexes (PRV-ISCOMs) were prepared by integrating envelope proteins of pseudorabies virus(PRV) into QuilA matrix, and its immunogenicity in mice was detected by ELISA,Neutralization test (NT) and proliferation of lymphocyte test (Brdu-ELISA assay). The results indicated: 1)The mice inoculated with 3, 7, 10ug of PRV-ISCOMs respectively developed ELISA IgG antibody 7 days post-inoculation, and had better antibody response after second inoculation interval 21 days. The neutralizing antibody titer (PD50)of mice after second inoculation reached above 1:22 and that of controls was negative. 2) The results of Brdu-ELISA indicated primarily that the mice vaccinated with PRV-ISCOMs can induce stronger cellular immune response than the controls. 3) The mice inoculated with PRV-ISCOMs obtained complete protection when challenged with PRV- FA 20 days after second inoculation. These results showed that PRV-ISCOMs can elicited excellent humoral and cellular immune response.更多还原