【摘要】 猪细小病毒 (PPV)强毒 ,在 1～ 5日龄仔猪肾原代细胞上传代 ,传至 2 0代左右毒力下降。为保持PPV强毒的毒力 ,我们用一头妊娠母猪 (妊娠 4 8d)做剖腹术。用大剂量的PPV细胞培养物注入猪胎儿的羊膜腔内 ,人工感染猪胎儿 ,病毒通过猪胎儿体内的组织进行复制 ,使毒力增强。术后 12d迫杀母猪 ,取胎儿分离PPV强毒。更多还原

【Abstract】 Porcine Parvovirus virulence was decreased when it was passed 20 generations in 1～5 day porcine kidney primary cell.We challenge adapted cell culture viruses into fetus amniotic cavity by the ventrotomy pregnant sow (pregnant 48 days),after challenged 12days,pregnant sow was slaughtered and viruses were isolated from infectious fetus.We made PPV virulence stronger by such means.更多还原