【摘要】 以猪肺炎支原体 (Mycoplasmahyopneumoniae,Mhp) 16 8菌株F332作为抗原 ,免疫 8周龄BALB/c小鼠 ,利用淋巴细胞杂交瘤技术 ,获得两株能稳定分泌特异单克隆抗体的杂交瘤细胞株。两株单抗与Mhp和猪鼻支原体 (M .hyorhinis ,Mh)等反应 ,而不与鸡支原体、对照血清等反应。结果表明两株单抗可能是针对猪支原体共同抗原的单抗。用SDS_PAGE电泳和Western_blot分析猪支原体的膜蛋白成分 ,结果显示 ,猪支原体的共同抗原是 80kd和 30kd蛋白 ,两株单抗均与Mhp和Mh的 30kd蛋白条带反应 ,表明这两株单抗特异地针对猪支原体的共同抗原成分 30kd蛋白。可以看出 ,这两株单抗在Mhp的抗原分析、血清学诊断和疫苗质量监测以及猪源支原体污染细胞检测等方面有重要应用价值更多还原

【Abstract】 week_old female BALB/c mice were immunized with the membrane protein of Mycoplasma hyopneumoniae (Mhp). By using the hybridoma technique, two hybridoma cell lines secreting monoclonal antibodies (MAbs) were established. Two MAbs could react with Mhp and M. hyorhinis (Mh), but neither could bind with avian mycoplasmas nor with control serum samples. The results suggested that two MAbs were probably specific to the common antigen of porcine mycoplasmas. By SDS_PAGE and western blotting analysis, it was shown that the main common antigens of porcine mycoplasmas were 80Kd and 30Kd proteins. Two MAbs specifically recognized the common antigen with 30Kd of porcine mycoplasmas in western blotting analysis. All these results indicated that these MAbs would be very useful as a tool for the antigenic analysis of Mhp, the serological diagnosis of Mhp, surveillance of vaccine products, and also detection of porcine mycoplasma contamination in cell cultures.更多还原