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Ⅰ、Ⅱ型糖尿病和透析患者的肠道内环境与生化指标的比较研究
INVESTIGATED THE INTESTINAL FLORA OF PATIENTS WHO ARE IN TYPE Ⅰ、Ⅱ DIABETES MELLITUS AND THE PERSONS UNDERGOING DIALYSIS
【摘要】 对Ⅰ、Ⅱ型糖尿病及透析患者的肠道内环境和血液指标进行了比较观察。肠内细菌群的检测采用光冈复合式法 ;氨和硫化物的测定 ,采用Terada的方法 ;吲哚及臭素等的测定方法采用同吉原方法。临床主要生化指标观察方法 ,采用美国康宁 6 4 4电解质分析仪和美国RA 10 0 0全自动生化分析仪。随机选取住院的Ⅰ、Ⅱ型糖尿病以及透析患者各 10名对肠道内环境以及血离子、肾功能进行检测分析。结果表明 :(1)肠内细菌群变化 :Ⅰ型糖尿病患者 ,双歧杆菌数为 7 73± 0 4 4 (logCFU/ g) ,占肠内总菌数的百分率为 (0 84± 0 75 )‰。腐败菌数亦为 8 14± 0 37。Ⅱ型糖尿病患者 ,双歧杆菌数为 7 88± 0 34(logCFU/g) ,占肠内总菌数的百分率为 (2 4 0± 3 18)‰。腐败菌数亦为 7 99± 1 15。透析患者 ,双歧杆菌数为 7 76± 0 4 2 (logCFU/ g) ,占肠内总菌数的百分率为 (0 78± 0 92 )‰。腐败菌数亦为 8 33± 0 5 0。并检测到绿脓杆菌 ,其检出率为 4 0 %。 (2 )腐败物质的变化 :Ⅰ型糖尿病患者粪便中氨为 82 3± 6 7 2 (μg/ g) ;硫化物为5 0 7± 16 0 ;粪便中苯、甲酚、吲哚、粪臭素等的变化 ,吲哚、粪臭素分别为 5 7 1± 12 1、5 3 5± 11 2 (μg/g)。Ⅱ糖尿病患者粪便中氨为 75 9 9± 6 2 9(μg/ g) ;硫?
【Abstract】 In this studies,the intestinal flora of 10 patients who are in type Ⅰ(Abb:ⅠDM)?Ⅱ(Abb:Ⅱ DM) diabetes Mellitus and the persons undergoing dialysis(Abb:DL) was investigated by Mitsuoka’s methods,and the putrefactive metabolites of those patients were analyzed by Terada’s methods,meanwhile,blood biochemical parameters was inspected by auto-biochemical analyzer.The results show that,the Bifidobacterial percentages in total fecal flora was 0.84±0.75 ammonia of Ⅰ DM and Ⅱ DM?DL was 823±67.2(μg/g) and 759.9±62.9?1 006.6±164.9.The urea was 7.75±2.29 (mmol/L) and 5.24±2.11?44.2±10.50 of Ⅰ DM and Ⅱ DM?DL.The creatinine of Ⅰ DM and Ⅱ DM?DL was 120.1±62.91(mmol/L) and 93.8±6.15?702.32±164.98.The uric acid was 0.29±0.04 (mmol/L) and 0.27±0.04?0.43±0.13 in Ⅰ DM and Ⅱ DM?DL.
【Key words】 Intestinal enviroment; Biochemical parameters; Diabettes Millus; Dialzer;
- 【文献出处】 中国微生态学杂志 ,Chinese Journal of Microecology , 编辑部邮箱 ,2003年04期
- 【分类号】R446.1
- 【被引频次】11
- 【下载频次】177