【摘要】 目的 :在C57BL/ 6小鼠建立MBP 特异性T细胞系 ,并探讨其生物学特性。方法 :取MBP免疫的C57BL/ 6小鼠腹股间淋巴结的淋巴细胞在体外培养 ,用MBP及同系小鼠的脾细胞反复刺激 ,建立了MBP 特异性T淋巴细胞。以流式细胞仪检测其细胞表面标志 ,以ELISA双抗体夹心法检测MBP 特异性T细胞产生的IFN γ及IL 4水平。结果 :CD4 + T细胞占 90 %以上。MBP 特异性T细胞主要产生IFN γ。结论 :该T细胞系为CD4 + 、Th1类淋巴细胞 ;在C57BL/ 6小鼠体内存在能识别MBP的自身反应性T细胞更多还原

【Abstract】 Objective:To establish MBP specific T cell line from C57BL/6 mouse,and investigate its biological characteristics.Methods:MBP specific T lymphocytes were established from inguinal lymph node of C57BL/6 mouse immunized with MBP and cultured in vitro might be classified stimulated with MBP and allogenic APCs for several times.The MBP specific T cells were identified by FCAS,the level of IFN  and IL 4 were assayed by ELISA.Results:More than 90% T 1ymphocytes were CD 4 +T cell and mainly produced IFN  Conclusion:The MBP specific T cell line belonged to CD 4 + Th1 lymphocytes更多还原