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UV-B辐射增强对水稻多胺含量及其相关酶活性的影响

Effect of enhanced UV-B radiation on polyamine contents and the activity of related enzymines in rice (Oryza sativa L.)

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【作者】 郭玉春林文雄吴杏春梁康迳梁义元

【Author】 GUO Yu-Chun,LIN Wen-Xiong,WU Xing-Chun,LIANG Kang-Jing,LIANG Yi-Yuan (College of Crop Science,Fujian Agricultural and Forestry University,Fuzhou 350002)

【机构】 福建农林大学作物科学学院福建农林大学作物科学学院 福州 350002福州 350002福州 350002

【摘要】 试验研究表明,处理前期(7~14d)UV-B辐射增强使“汕优63”水稻精氨酸脱羧酶(ADC)、鸟氨酸脱羧酶(ODC)和S-腺苷蛋氨酸脱羧酶(SAMDC)活性分别增加165.74%、104.60%和89.60%,“南川”(NC)运3种酶活性分别增加59.91%、41.30%和23.68%,新品系“IR65600-85”在UV-B辐射胁迫下多胺脱羧酶活性表现与前2品种略不同,即ADC和ODC活性分别提高115.93%、14.45%,而SAMDC活性下降33.01%。处理后期(21~28d)随UV-B辐射累积量的增加,这3种酶活性均有所下降,其中ODC和SAMDC活性降幅大于ADC,其中“汕优63”ADC、和ODC活性分别比对照增加89.72%、3.71%,“南川”则分别增加73.95%、27.38%,“IR65600-85”ADC活性增加94.41%,ODC、活性却下降13.57%。处理后期(21~28d)3类水稻“汕优63”、“南川”和“IR65600-85”SAMDC分别下降40.06%、19.20%和38.21%。多胺氧化酶(PAO)活性变化趋势则相反,即处理前期(7~14d)呈不同程度下降趋势,处理后期(21~28d)则呈极显著上升趋势,其结果引起多胺(PA)含量特别是腐胺(Put)含量明显上升。水稻对UV-B辐射增强的反应具有基因型差异。

【Abstract】 Experimental results show that when the plants are exposed to enhanced UV-B radiation for 7 ~ 14 days,the activities of agrininc decarboxylase (ADC),ornithine decarboxyiase (ODC) and s-adenosylmethionine decarboxylase (SAMDC) in leaves are increased by 165.74%,104.60% and 89.60% in "Shan You63" (Sy63),59.91%,41.30% and 23.68% in "Nancheum" (NC) and 115.93%,14.45% arid-33.01% in IR65600-85,respectively.However,the three enzymic activities turn to decrease with the treatment time increasing (21~28d) and the decreased extents of ODC and SAMDC are much higher than that of ADC,.The activities of ADC and ODC are increased by 89.72% and 3.71% in Sy63 and 73.95 % and 27.38 % in NC exposed to UV-B radiation for 21 ~ 28 days.The activity of ADC is also increased by 94.41% but ODC activity is decreased by 13.57% in the leaves of IR65600-85 compared with the controls.As far as SAMDC is concerned,the enzymic activities in Sy63,NC and IR65600-85 are reduced by 40.06%,19.20% and 38.21% respectively when they are exposed to enhanced UV-B radiation for 21~28 days.The reverse is true in the case of poly-amine oxidase (PAO),indicating its lower activity in earlier treatment courses (7~14d),but much higher activity in late courses (21~28d) compared with the controls.These in turn result in the increased contents of polyamine (PA),especially putrescine (Put) and the results of the experiment have shown their differences in diverse rice genotypes.

【关键词】 水稻UV-B辐射增强多胺代谢
【Key words】 RiceEnhanced UV-B radiationPolyaminc metabolism
【基金】 国家自然科学基金(39970441);教育部优秀年轻教师基金;福建省科技厅国际合作项目(20011010)
  • 【文献出处】 中国生态农业学报 ,Chinese Journal of Eco-Agriculture , 编辑部邮箱 ,2003年02期
  • 【分类号】S511
  • 【被引频次】23
  • 【下载频次】206
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