【摘要】 从东北地区发生新城疫 (ND)的病鸡、病鸽和产蛋下降鸡分离到 19株NDV ,对其融合蛋白 (F)基因 5 32bp或 2 80bp片段进行了克隆、测序 (在GenBank中的登录号为AY2 0 86 80～AY2 0 86 98) ,并对各株的F基因序列进行了比较。结果 ,各毒株之间的核苷酸同源性为 83.1%～10 0 % ,氨基酸同源性为 85 .5 %～ 10 0 % ;系统发育进化树分析表明 ,其中 2株与疫苗株V4同属基因Ⅰ型 ,为弱毒株 ;其余 17株为强毒株 ,其中 2株为基因Ⅵ型 ,其余为基因Ⅶ型。表明东北地区目前由 3种基因型的NDV毒株 (Ⅰ型、Ⅵ型、Ⅶ型 )所引发 ,并以基因Ⅶ型为主。另外 ,对其中的代表毒株APMV1/chicken/China/JL 11/ 0 2进行了免疫学试验 ,结果显示 ,LaSota疫苗的免疫保护效果不理想 ,可能是LaSota疫苗免疫鸡群发生ND的主要原因。更多还原

【Abstract】 19NDV strains were isolated from sick chickens and pigeons or egg drop chickens in northeastern China. These strains were studied by RT-PCR and the sequences of the region of 532 or 280 nucleotides covering the fusion (F) protein cleavage site were analyzed (GenBank accession No.AY208680-AY208698). Nucleotide and predicted amino acid similarities of 19 NDV were 83.11%-100% and 85.5%-100% respectively. Phylogenetic tree of the nucleotide sequence demonstrated that 2 strains are lentogenic strains and shared genetype Ⅰwith vaccine strain V4, 17 strains are velogenic and 2 strains of them were genetype Ⅵ and the others were genetype Ⅶ. At the same time, the representational strain APMV1/chicken/China/JL-11/02 was evaluated by trial of immunity. The result revealed that LaSota vaccine can not give good protection. This is probably important reason of immunity lose with LaSota.更多还原