【摘要】 参照GenBank中收录的鹅细小病毒 (GPV)B株基因序列设计并合成了扩增GPVH1株VP1的 1对引物 ,利用PCR技术扩增出长约 2 .2kb的目的片段 ,将其克隆到 pMD 18 T载体上 ,进行了序列测定及分析。测序结果表明 ,GPVH1株VP1基因由 2 199个核苷酸组成 ,编码 732个氨基酸。经与B株、YG株进行同源性比较 ,核苷酸的同源性分别为 98.5 0 %和 93.18% ;推导的氨基酸同源性分别为 98.0 9%和 95 .77%。更多还原

【Abstract】 A pair of primers was designed to amplify VP1 gene of GPV by PCR according to the published sequence of GPV B strain in GenBank. The product of PCR whose length is 2.2 kb was cloned to the pMD 18-T vector,then made sequencing and analyzing . The result of sequence showed that VP1 gene (included) 2 199 bp, which encoded 732 amino acids. The gene shared 98.50% and (93.18%) homology in (bases), and shared 98.09% and 95.77% respectively in amino acid with GPV strains B and YG.更多还原