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鸡传染性喉气管炎病毒gB基因的扩增及酶切分析
Amplification of infectious laryngotracheitis gB gene by PCR and analysis of its product by 3 kinds of restriction endonucl eases
【摘要】 以从内蒙古区分离的 2株传染性喉气管炎病毒 (ILTV)株的DNA为模板 ,用设计好的 1对引物对这 2株毒株的 gB基因进行了PCR扩增 ,并对扩增产物分别用BglⅡ、PstⅠ、HindⅢ限制性内切酶酶切分析。结果表明 ,2个分离株均能特异性地扩增出该病毒 gB基因片段 ,片段大小完全一致 ,为 2 .6kb ;扩增产物的酶切图谱与参考株完全一致。
【Abstract】 On the template DNA from 2 avian infectious lar yn gotracheitis virus (ILTV) strains isolated from Nei Monggol Region of China, ILT V gB gene fragments were amplified with a pair of designed primers by polymerase chain reaction. Restricti on analysis of the mentioned-above amplification products was carried out with BglⅡ,PstⅠ and HindⅢ. The resu lts showed that length of the amplification products of the strains is 2.6 kb, a nd their restriction maps are identical to that of the prototype strain.
- 【文献出处】 中国兽医科技 ,Chinese Journal of Veterinary Science and Technology , 编辑部邮箱 ,2003年08期
- 【分类号】S852.65
- 【被引频次】1
- 【下载频次】86