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NSP-ELISA鉴别FMDV感染与免疫

Differentiating infection from vaccination against foot-and-mouth disease by a non-structural protein ELISA

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【作者】朱彩珠；张强；常惠芸；卢永干；邱孝高；尤永进；葛艳；陈波；饶忠；徐泉兴；

【Author】 ZHU Cai-zhu 1, ZHANG Qiang 1, CHANG Hui-yun 1, LU Yong-gan 1, QIU Xiao-gao 1, YOU Yong-jin 2, GE Yan 2, CHEN Bo 2, RAO Zhong 2, X U Quan-xing 2 (1. Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Scien ces, Lanzhou 730046,China; 2. Animal Science and Veterinary Medicine Research Institu te, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China)

【机构】中国农业科学院兰州兽医研究所；上海市农业科学院畜牧兽医研究所；上海市农业科学院畜牧兽医研究所甘肃兰州730046；甘肃兰州730046；上海201106；上海201106；

【摘要】利用大肠埃希氏菌表达的口蹄疫病毒 (FMDV)非结构蛋白 (NSP) 3ABC多肽作为抗原 ,建立了一种可区分FMDV感染与接种灭活疫苗免疫牛的间接酶联免疫吸附试验 (I ELISA)。通过对 336份正常牛血清、50 8份注射疫苗牛血清、60份人工感染牛血清和 58份豚鼠免疫血清的检测 ,确定了血清抗体阴性与阳性效价的临界值。试验证明 ,该方法简易、快速 ,灵敏度比VIAA AGID高更多还原

【Abstract】 An indirect ELISA was developed for differenti at ion of infection from vaccination against foot-and-mouth disease virus (FMDV) using a non-structural protein 3ABC as antigen expressed in Escherichia coli. The negative and positive cut off values for absorbances of the I -ELISA were established according to the results by detection of 962 serum samples, that included 336 sera from native cattles, 508 from vaccinated cattles , 60 from infected cattles, and 58 from hyperimmune cavians. The I-ELISA test i s easier to operate, quicker to obtain result, and more sensitive than the VIAA -AGID.更多还原

【关键词】口蹄疫病毒；非结构蛋白；间接酶联免疫吸附试验；
【Key words】 foot-and-mouth disease virus； non-structural prote in； indirect-ELISA；

【基金】上海市科学技术发展项目 (2 0 0 0 0 0 34130 0 9)

【文献出处】中国兽医科技 ,Chinese Journal of Veterinary Science and Technology , 编辑部邮箱 ,2003年08期

【分类号】S852.65
【被引频次】33
【下载频次】152

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