【摘要】 根据已发表的减蛋综合征病毒 (EDSV)核苷酸序列设计了 1对扩增长度为 1 .1kb左右的引物 ,采取降落PCR对EDSV gDNA进行扩增。结果 ,从 2份EDSV鸭胚尿囊液、3份人工感染鸡输卵管组织液的核酸抽提物中 ,均扩增出与设计大小完全一致的目的片段 ,而对照样品的扩增均为阴性 ,该方法最低可检测到 32pg的EDSV gDNA。表明该降落PCR用于检测EDSV特异性和敏感性均较强 ,可用于减蛋综合征的诊断更多还原

【Abstract】 A pair of primers were designed according to the sequences of PVⅢ gene of egg drop syndrome virus (EDSV). Using the primers, the PVⅢ gene was amplified by touchdown PCR (TD PCR). The results showed that the target fragments were amplified from 2 allantoic fluid samples of EDSV and 3 tissue samples of infected chickens, but the PCR products of control sample were negative. The method can detect as little as 32pg EDSV gDNA. It can be concluded that the TD PCR was a specific and sensitive highly dignosis method to EDSV.更多还原