【摘要】 从电脉冲次数、脉冲强度、脉冲间隔时间三方面对兔卵母细胞的孤雌激活进行了研究。结果表明 ,注射HCG后 16h的MⅡ期卵 ,用 1.4kV/cm、6 0 μs的直流脉冲电激 ,脉冲次数分为 1、2、4、8次 4组 ,每次间隔 30min ,随着电激活次数的增加 ,活化率从 4 6 .5 %增加到 95 .8% (P <0 .0 1) ,但是电激活 8次后 ,桑椹胚率却从 6 3.6 %下降到 2 1.2 % ,囊胚率从 2 3.6 %下降到 2 .8%(P <0 .0 1) ;用 6 0 μs的直流脉冲电激 3次 ,每次间隔 30min ,分为 0 .4、1.2、2 .4kV/cm 3组 ,以1.2kV/cm脉冲强度的兔卵母细胞激活较为理想 ,脉冲强度增加到 2 .4kV/cm时 ,卵细胞死亡率升为6 7.1% (P <0 .0 1)。用 1.4kV/cm、6 0 μs的直流脉冲电激 3次 ,电脉冲间隔时间分为 30、12 0、2 4 0min 3组 ,卵细胞活化率分别为 6 8.6 %、91.5 %、91.9% (P <0 .0 1)。更多还原

【Abstract】 Activation of MⅡ stage rabbit oocytes was studied here. By using 1.4 kV/cm, 60μs electrical pulse, the effect of 1,2,4,8 treatments over 30 min upon oocytes activation were studied. The results showed that with the increasing electrical pulse treatment, the rates of activation increased from 46.5% to 95.8% (P<0.01), the rates of morula and blastocyst decreased sharply with extended eight pulses treatment duration (morulae: from 63.6% to 21.2% and blastocysts: from 23.6% to 2.8%, P<0.01) . By using 60 μs, 3 treatment electrical pulses, the effect of 0.4 kV/cm, 1.2 kV/cm, 2.4 kV/cm voltage, over 30 min upon oocytes activation were studied. The results showed that 1.2 kV/cm was the suitable voltage, the rate of death of oocytes increased from 0 to 67.1% with the 2.4 kV/cm treatment.By using 1.4 kV/cm , 60 μs, 3 treatment electrical pulses ,the effect of over 30,120,240 min upon oocytes activation were also studied. The results showed that the activation rates were 68.6%, 91.5% and 91.9% respectively (P< 0.01).更多还原