【摘要】 目的 :探讨睫状神经营养因子 (CNTF)对N 甲基 D 天冬氨酸 (NMDA)引起大鼠海马神经元蛋白激酶C(PKC)发生核转位的影响。方法 :以NMDA及CNTF处理原代培养的大鼠海马神经元 ,PKCγ、PKCε免疫细胞化学并结合图象分析方法测定PKC阳性神经元胞核的灰度。结果 :①给予不同浓度NMDA处理不同时间后 ,神经元核内有不同程度的PKCγ及PKCε表达 ,其中以 10 0 μmol/LNMDA 30min组表现尤为显著 ;②CNTF +5 0 0 μmol/LNMDA组神经元胞核PKC灰度与对照组相似。结论 :NMDA可引起海马神经元PKCγ、PKCε的核转位 ,而CNTF则抑制其转位的发生 ,提示CNTF对海马神经元的保护作用与抑制PKC的核转位有关。更多还原

【Abstract】 Aim: To investigate the effect of ciliary neurotrophic factor (CNTF) on the nuclear translocation of protein kinase C (PKC) following NMDA administration in the primary cultured hippocampal neurons. Methods: ① PKCγ or PKCε- immunocytochemistry staining method was used after treating neurons with NMDA or CNTF. ② The gray of the nucleus of the PKC-positive neurons were measured under the image pattern analysis system. Results: ① After NMDA administration of different concentration and time, Nucleus appear PKCγ and PKCε activities, especially the 100 μmol/L NMDA 30 min group. ② The gray of nucleus in CNTF + 500 μmol/L NMDA group is similar to control group. Conclusion: NMDA can induce nuclear translocation of PKC in the primary cultured hippocampal neurons, and CNTF can inhibit the translocation. It suggests that the inhibition of PKC translocation induced by NMDA is one of the important reasons for the neuro-protective effects of CNTF.更多还原