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水稻中花11辐射突变体的分离与鉴定
Screening and Characterization of Mutants Induced from Zhonghua 11 (Oryza sativa L. subsp. japonica) by Irradiation
【摘要】 利用伽玛射线对粳稻品种中花 11进行辐照 ,以诱导广泛的形态突变体 ,为水稻功能基因组研究提供基础材料。辐射第 1代单本移栽并实行单株收获 ;辐射第 2代 (M2 )播种 1万个家系 ,自苗期开始至成熟期各个生育阶段从中独立选获各类形态突变体 5 40个 ;经M3 复选获得包括叶、茎、穗和育性变化等各类突变体 431份。统计结果表明 ,形态性状的突变频率为 5 .1%。
【Abstract】 The japonica rice (Oryza sativaL.) variety Zhonghua 11, developed by anther culture technology, was irradiated by gamma rays to induce extensive mutation for functional genomics research use. M 1 generation was transplanted and harvested by each single plant. Ten thousand families of M 2 generation were planted. From seedling stage to maturity stage in M 2 generation, 540 morphological mutants were isolated. In M 3 generation 431 mutants including leaf, stalk, panicle and sterility variations were identified and selected repeatedly. The statistic analysis indicated that the mutation frequency of morphological character was 5.1%.
【Key words】 Oryza sativa; irradiation; mutant; morphological characteristics;
- 【文献出处】 中国水稻科学 ,Chinese Journal of Rice Science , 编辑部邮箱 ,2003年03期
- 【分类号】S511.22
- 【被引频次】52
- 【下载频次】259