【摘要】 目的　建立SPF级湖北封闭群KM小鼠生产群。方法　使用超净工作台实施子宫摘除术净化小鼠的方法 ,建立了SPF级湖北封闭群KM小鼠生产群 ,并对子一代进行了微生物及寄生虫的检测。结果　使用上述方法实施手术 6 7例 ,成功 5 7例 ,得净化仔鼠 5 0 9只 ,育成的 4 6 0只小鼠符合SPF级标准。结论　使用生物净化的方法可以保存优良实验动物品种。更多还原

【Abstract】 Objective To establish special pathogen free Kunming mouse of Hubei closed colony.Method The purification mouse was made by excising operation of uterus in the situation of bacteria free. The SPF Kunming mouse strain of Hubei closed colony was developed with the following surveillance on microbes and parasites in first generation.Results 57 mice were got from 67 cases by operation, and 509 baby mice with purification,from which 460 grew into adults and the state of microbiology in those mice was in keeping SPF.Conclusion The method can keep the excellent laboratory animal stain with biological purification.更多还原