【摘要】 目的:研究尼莫地平与甘露醇联合疗法对大鼠脑缺血再灌注的的保护作用。方法:60只大鼠随机分为治疗组、对照组和正常组,采用线栓法制作大鼠脑缺血再灌注模型,治疗组给予尼莫地平与甘露醇联合疗法处理。在再灌注1d、3d检测脑组织SOD活性及MDA含量,并用免疫组织化学方法检测脑内神经生长和修复相关蛋白GAP-43、MAP-2和cyclinD1的表达情况。结果:脑缺血再灌注后SOD活性降低,MDA含量增高(P<0.05),神经组织呈破坏性改变,缺血灶周围GAP-43、MAP-2和cyclinD1表达增高(P<0.01),治疗可减轻脑病理损害,并增强GAP-43和MAP-2的表达(P<0.01),抑制cyclinD1的表达。结论:尼莫地平与甘露醇联合疗法可减轻水肿,清除自由基,抑制细胞凋亡和蛋白水解,对缺血脑组织有明显的保护作用,并促进损伤神经组织的修复。更多还原

【Abstract】 Objective:To investigate the effect of nimodipine combined with mannitol therapy on rats that suffered from cerebral ischemia reperfusion injury.Method:60rats were divided randomly into3groups,treatment group and control group,each containing24rats,and12rats in normal group.Rats in treatmnet group accepted nimodipine combined with mannitol therapy after middel cerebral artery occlusion(MCAO)for one hour,while rats in control group accepted no treatment after ischemia reperfusion injury.One or three days later,rats were decapitated and their brains were extracted for SOD activity and MDA concentration assay.Immunohistochemistry was used to e鄄valuate the expression of GAP-43,MAP-2and cyclin D 1 protein.Result:SOD activity decreased in rats after is鄄chemia reperfusion injury while MDA concentration increased,compared with normal rats(P<0.05).And also,level of GAP-43and MAP-2expression in rats undergone operation was lower than that of those not(P<0.01),while level of cyclin D 1 expression was higher than normal(P<0.01).Contrasted to control group,SOD activity in treatment group was higher and MDA concentration was lower.On the hand of immunohistochemistry,level of GAP-43and MAP-2expression in treatment group was higher than that in control group,while level of cyclin D 1 was lower.Differences between treatment group and control group were significent.Conclusion:Nimodipine combined with man鄄nitol therapy can prevent brain from ischemia reperfusion injury.Its actions of wiping free radicals out and pro鄄moting nerve regeneration are involved in the mechanism.更多还原