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一株鹅源呼肠孤病毒的分离与鉴定

Isolation and identification of one goose reovirus strain

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【作者】 王光锋; 王永坤; 王建业; 朱国强; 严维巍; 周继宏; 庄国宏; 钱钟;

【Author】 Wang Guangfeng,Wang Yongkun,Wang Jianye,Zhu Guoqiang,Yan Weiwei,Zhou Jihong,Zhuang Guohong,Qian Zhong(College of Animal Husbandry and Veterinary Medicine,Yangzhou University,Yangzhou,Jiangsu225009)

【机构】 扬州大学畜牧兽医学院; 扬州大学畜牧兽医学院 江苏扬州225009; 江苏扬州225009; 江苏扬州225009;

【摘要】 一株从雏鹅肝脾脏病料中分离到的病毒,在电镜下观察到病毒粒子呈球形,无囊膜,有可见的双层衣壳结构,外壳直径75nm,内核直径50nm;经病毒分离培养、理化特性鉴定、动物回归试验、抗体检测,确定所分离到的毒株为呼肠孤病毒科正呼肠孤病毒属鹅呼肠孤病毒。

【Abstract】 One virus strain was isolated from the liver and spleen of gosling.The virion showed spherical,no enve-lope,having double-deck capsids,with outer-capsid of75nm in diameter and inner-capsid of50nm respectively by TEM.By virus-isolation,identifying physicochemical properties,experimental infection,antibody detection and electromicro-scope ob servation,This strain was identified as one member of the family reoviridae,genus Orthoreovirus,goose re ovirus.

【关键词】 鹅; 呼肠孤病毒; 分离; 鉴定;
【Key words】 gosling; reovirus strain; isolation; identification;
  • 【分类号】S858.33
  • 【被引频次】44
  • 【下载频次】228
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