【摘要】 用HPLC方法提纯经雌二醇诱导的鲫鱼卵黄蛋白原,用SDS-PAGE凝胶电泳测定其分子量为160Kda左右.在建立卵黄蛋白原(VTG)的HPLC分析方法(检测下限为0.025mg/mL)的基础上,对北京排污河天津段的野生和养殖鲫鱼血浆中的VTG进行检测.在所捕获的12尾野生鲫鱼中(其中2尾为雄性鲫鱼),都检测出了VTG,浓度范围为0.284~5.971mg/mL;对同期捕获的35尾天津养殖鲫鱼包括1尾雄性鲫鱼中的VTG也进行了检测,浓度范围为0.119~0.250mg/mL.而同期在实验室饲养的雌雄鲫鱼血浆中,均未检测出VTG.结果表明,北京排污河(天津段)的野生鲫鱼受到类雌激素物质的污染.更多还原

【Abstract】 Vitellogenin (VTG) in crucian (Carassius auratus) induced by injection of 17?estradiol was purified using HPLC method, and its molecular weight was determined to be about 160Kda using SDS-PAGE. Based on the HPLC analysis method of vitellogenin established (its low limit of detection was 0.025靏/mL), the VTG in the plasma of wild and cultured crucians from Beijingpaiwu River in Tianjin area was detected. Vitellogenin was detected in all of 12 caught wild fishes, (including 2 male fishes) and the concentration ranged from 0.284 to 5.971mg/mL. Vitellogenin was also detected in all of 35 cultured fishes including 1 male, catched in same period and the concentration ranged from 0.119 to 0.250mg/mL; but no vitellogenin was detected in both male and female cultured in laboratory in same period. The wild crucian from Beijingpaiwu River in Tianjin was contaminated by estrogenic matter.更多还原