【摘要】 目的　研究镉对去卵巢大鼠钙代谢的影响。方法　将SD大鼠行人工去卵巢术 ,分别经饮水给不同剂量(5 0 ,10 0 ,2 0 0mg/L)的镉染毒 2 4周。分别于第 6,12 ,18,2 4周收集 2 4h尿液、粪便。用石墨炉原子吸收法测定血、尿镉 ,放射免疫法测定血清雌二醇、尿 β2 微球蛋白 ,同时测定粪、尿及骨钙含量 ,计算粪钙及尿钙日排泄总量。结果　大鼠去卵巢后 ,体内雌二醇明显下降。镉染毒后 ,去卵巢大鼠粪钙及尿钙日排泄总量明显升高 ,且呈剂量 反应关系。 2 0 0mg/L镉染毒组去卵巢大鼠的骨钙含量也显著性下降。同时镉染毒的去卵巢大鼠发生肾损害 ,表现为尿 β2 微球蛋白排泄增加。尿钙日排泄总量与β2 微球蛋白显著性正相关。结论　镉可导致去卵巢大鼠钙代谢损害 ,肠钙及尿钙的排泄量增加 ,使骨钙含量降低更多还原

【Abstract】 Objective To investigate effects of cadmium on calcium metabolisms of ovariectomized rats.Methods Female Sprague Dawley rats aged 6 months were ovariectomized.The rats were administrated with cadmium at the dose of 0,50,100 and 200 mg/L in drinking water for 24 weeks.Urine and feces of 24 hours were collected after 6,12,18 and 24 weeks,respectively.Cadmium concentration in urine,blood and bone were measured by an atomic absorption spectrophotometer.And urinary β\-2 MG and serum estradiol were detected by RIA.The calcium contents in bone,urine and feces were assayed.Results Compared to the sham operation group,estradiol significantly declined in the ovariectomized rats.After cadmium administration,the excretions of calcium in feces and urine increased significantly in a dose response related pattern.And calcium contents of bone were significantly lower in the group exposed to 200?mg/L cadmium in drinking water than in the control group.Urinary β\-2 MG was significantly higher in cadmium exposed groups.Meanwhile urinary β\-2 MG positively related to total excretions of calcium in 24 hours.Conclusion The results indicated that cadmium could inhibit calcium metabolisms by increasing the excretions of calcium in feces and urine,which probably resulted in significant calcium declines in bone.更多还原