【摘要】 目的　建立恒河猴子宫内膜炎性出血模型。方法　选择月经正常的雌性育龄恒河猴 7只 ,随机分为实验组 4只和对照组 3只。实验组在月经第三天 ,用致病性大肠埃希菌、金黄色葡萄球菌、厌氧消化链球菌混合感染恒河猴的子宫内膜 ,宫腔细菌接种后第 5天进行宫颈分泌物的细菌培养鉴定 ,继而连续观察三个月经周期经量、经期等的变化 ,于第四个月经周期分泌晚期取子宫内膜进行组织病理学检测。对照组未进行宫腔细菌接种 ,其经量、经期的观察及内膜取材时间同实验组。结果　①实验组宫颈分泌物细菌培养鉴定证实三种细菌为本实验宫腔接种的细菌。②实验组与对照组比较 ,月经量明显增多、经期明显延长。③光镜观察显示 :实验组为慢性子宫内膜炎症改变 ,表现为内膜上皮坏死 ,间质炎性细胞增多明显 ,以中性粒细胞为主 ,间质出血严重 ,表浅微血管、静脉窦扩张明显 ,螺旋动脉扩张较明显 ,特别是在扩张的、大量炎性分泌物的腺体周围。其内皮细胞及平滑肌细胞变性严重、结构模糊、界限不清 ,平滑肌细胞排列紊乱。对照组为正常行经前期图像 ,螺旋动脉强烈收缩 ,内皮细胞无变性。结论　通过月经情况的改变及内膜形态学观察评价该模型是成功的。更多还原

【Abstract】 Objective To establish metrorrhagia animal model induced by endometritis in experimental Rhesus monkey. Methods Seven normal menorrhea adult %Rhesus monkeys% were choosed and divided into experimental group and control group at random. Escherichia coli,%staphylococcus aureus% and %peptostreptococcus% were inoculated into uterine cavity of experimental group in the third day of menstruation. Then the cervical secretions were cultured and evaluated in the fifth day of inoculation and the changes of menstrual amount and duration were observed in three continuous menstrual cycle. The sample of endometrium was obtained in late secretory phase of the fourth menstrual cycle for examination under microscope. Endometrium of Control group was not infected, the other procedures were the same as experimental group. Results 1.The evaluation of cervical secretion culture and confirmed that the three kinds of bacteria were inoculants in the experimental group. 2.Compared with control group, experimental group was more excessive in menstrual amount and much longer in duration. 3. Compared with control group, endometrium of experimental group showed the change of chronic inflammation, in which the degeneration of spiral arteriolar wall was more severe and dilatation of lumen more obvious. Conclusion the model comes true by evaluation of the menstrual change and endometrium morphology observation.更多还原