【摘要】 目的　建立湖北封闭群日本大耳白兔生物学数据库 ,为毒理学实验提供相关的实验动物背景资料。方法　采用物理称重法测定日本大耳白兔体重、脏器重量 ;自动血球计数仪、半自动生化分析仪测定红细胞数等 10项血液有形成分和血红蛋白 ,以及碱性磷酸酶等 10项生化参数。并与国内常用新西兰和北京的大耳白实验兔有关参数进行对比。结果　提供了湖北封闭群日本大耳白兔的体重、脏器重量 ;RBC ,HGB ,MCV ,MCH ,MCHC ,HCT ,PLT ,MPV ,WBC ,W SCRW LCR ,和AST、ALT、ALP、BUN、TP、ALB、GLU、T -BIL、Grea、T -CHO等与安全性评价有关常用参数。结论　不同品种间的动物生物学特性有差异 ,即使同一品种而地区不同的封闭群动物 ,由于环境、饲养方式、营养状态不同 ,某些生物学特性和血液、生化参数也有差别。因此 ,为毒理学实验者提供实验家兔时 ,除提供微生物等级合格证外 ,还应按实验需求提供有关的生物学参数。更多还原

【Abstract】 Objective To establish the data bank of biological parameters of Hubei closed colony Japanese white rabbits for providing biomedical toxicology experiments with relative data of laboratory animal.Methods The rabbit body weight and organ weight were measured. The some of haematological parameters such as RBC, HGB ,MCV, MCH, MCHC, HCT, PLT, MPV, WBC, W-SCR W-LCR were examined. The biochemical parameters of AST,ALT,ALP,BUN,TP,ALB,GLU,T-BIL,Grea,T-CHO were detected. Those were compared with the parameters of common experimental rabbits. Results and Conclusion All the above mentioned parameters were obtained. The biological characters were different among the different species of rabbits, even in same species, and different biological characters due to different regions, environments, breed statuses and nutrition.更多还原