【摘要】 目的　研究灰旱獭感染HBV的传代实验。方法　对人工饲养两个月后的健康、HBV本底阴性的灰旱獭 ,接种含HBV DNA的人血清 ,接种后 1～ 4周 (每周 ) ,2～ 8个月 (每月 )查HBV M—ELISA法 ,HBV DNA—PCR ELISA ,ALT AST检测同步取肝组织做病理检查。用产生应答的阳性血清同法依次进行第二代、第三代实验。结果第一、二代血清和肝组织炎症反应均为阳性 ,第三代血清无应答 ,但肝组织呈现炎症反应。结论　初步证实灰旱对HBV攻毒、传代成功。更多还原

【Abstract】 Objective To study HBV-infected grey marmot (marmota baibacina) generation after generation.Methods The healthy and HBV-infected marmots,which bred in the laboratory more than two monthes,were injected with the human serum containing HBV-DNA.HBV-M,HBV-DNA,and ALT/AST were observed by ELISA,PCR-ELISA,Lai’s methods each week from the frist to fouth week and each month from the second to eighth month.The relative tissues were checked on pathology.The second and third generations were injected in the same way with the mixture serum,which came from the postive bodies in the first generation infected by HBV.Results\ There were good responses of the serum in the first and second generation,but no response of the serum in the third generration.Inflammatory responses of liver tissue could be found in all generations. Conclusion\ The preliminary study confirmed that it would be hopeful for grey marmot to be animal model with HBV.更多还原