【摘要】 目的 :肝癌高低转移细胞株的基因表达谱的比较。方法 :应用cDNAmicroarray技术 ,对肝癌高低转移细胞株的基因表达谱进行比较 ,并应用RT PCR对部分差异表达的基因进行了验证。结果 :肝癌高转移细胞株与低转移细胞株相比 ,有 384个基因存在差异表达 ,其中 1 2个是位于染色体 8p上。选取 4个基因用RT PCR验证 ,结果与cDNAmicroarray结论基本一致。 结论 :肝癌高低转移细胞株基因表达存在明显差别。染色体 8p上可能存在促进和抑制肝癌转移的基因更多还原

【Abstract】 Purpose:To study the difference in gene expression profile between HCC cell strains with high and low metastatic potential.Methods:The gene expression profile of high and low metastatic HCC cell strains were analyzed by cDNA microarray, and genes with differential expression were further verified by RT PCR.Results:384 differentially expressed genes were detected between high and low metastatic HCC strains, making up 32% of the total genes studied. RT PCR analysis on 4 genes chosen demonstrated the same results as those revealed by the array technique.Conclusions:Obvious difference in gene expression patterns was observed between high and low metastatic HCC cell strains, chromosome 8p may contain metastasis promote and inhibitory genes.更多还原