【摘要】 目的 :研究非小细胞肺癌患者外周血T淋巴细胞活化相关膜分子的表达 ,进一步探讨肺癌的免疫功能。方法 :应用流式细胞双色免疫荧光标记技术对 50例肺癌患者外周血T淋巴细胞活化相关膜分子CD3 PE和CD2 5 HLA DR CD69 FITC表达进行荧光免疫检测 ,并与正常对照组 (n =2 5)进行对比研究。结果 :50例肺癌患者外周血T淋巴细胞中CD3+ CD2 5+ 、CD3+ HLA DR+ 和CD3+ CD69+ 表达显著低于正常对照组 (P <0 .0 1 )。手术后外周血CD3+ CD2 5+ 、CD3+ HLA DR+ 和CD3+ CD69+ 表达高于手术前 (P <0 .0 1或P <0 .0 5) ,化疗前后差异无显著性 ;肺癌伴淋巴结转移CD3+ CD2 5+ 、CD3+ HLA DR+ 和CD3+ CD69+ 低于不伴淋巴结转移者 (P <0 .0 1 ) ;Ⅲ期、Ⅳ期和Ⅰ期、Ⅱ期之间CD2 5、HLA DR和CD69表达比较 ,差异有显著性 (P <0 .0 1 ) ;CD3+ CD2 5+ 、CD3+ HLA DR+ 和CD3+ CD69+ 表达与肺癌组织学分级及年龄有明显相关性 (P <0 .0 5或P <0 .0 1 ) ;与鳞癌和腺癌及患者的性别没有相关性。结论 :应用流式细胞仪检测外周血CD3+ CD2 5+ 、CD3+ HLA DR+ 和CD3+ CD69+ 的表达水平对了解患者的免疫状态、病情判断、预后评估及进行免疫治疗具有重要的意义更多还原

【Abstract】 Purpose:To study the expression of T lymphocyte activation associated membrane molecule in peripheral blood of patients with non small cell lung cancer and study the changes in its immunity function.Methods:The lymphocyte from the peripheral blood in patients with non small cell lung cancer were immunologically double fluorescent labeled by CD3 PE and CD25/ HLA DR/ CD69 FITC were determined by flow cytometry. 25 normal donors served as controls.Results:Peripheral blood CD3 + /CD25 + ,CD3 + /HLA DR + and CD3 + /CD69 + in T lymphocyte with lung cancer were significantly lower than those in normal controls ( P <0.01). CD3 + /CD25 + ,CD3 + /HLA DR + and CD3 + /CD69 + in post operative T lymphocytes were significantly higher than pre operatively( P <0.01 or P <0.05),there was no significant differences between pre chemotherapy and post chemotherapy. Lung cancer with lymph node metastasis showed lower expression of CD3 + /CD25 + ,CD3 + /HLA DR + and CD3 + /CD69 + than lung cancer without lymph node metastasis ( P <0.01). Distinct statistic differences were observed between CD3 + /CD25 + ,CD3 + /HLA DR + and CD3 + /CD69 + in stage Ⅲ,Ⅳand stageⅠ,Ⅱ( P <0.01). Histopathological grade and age were also significantly associated with the expression of CD3 + /CD25 + ,CD3 + /HLA DR + and CD3 + /CD69 + ( P <0.01 or P <0.05). Peripheral blood CD3 + /CD25 + ,CD3 + /HLA DR + and CD3 + /CD69 + contents were not related with squamous carcinoma, adenocarcinoma and gender.Conclusions:Detection of CD3 + /CD25 + ,CD3 + /HLA DR + and CD3 + /CD69 + levels by flow cytometry might be helpful for reflecting the human immunity function, judgement of disease status, prognosis evaluation and immunological treatment in patients with non small cell lung cancer.更多还原