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T细胞活化相关膜分子在非小细胞肺癌外周血中的表达及其临床意义
T lymphocyte activation associated membrane molecule expression in peripheral blood of patients with non-small-cell lung cancer and its clinical significance
【摘要】 目的 :研究非小细胞肺癌患者外周血T淋巴细胞活化相关膜分子的表达 ,进一步探讨肺癌的免疫功能。方法 :应用流式细胞双色免疫荧光标记技术对 50例肺癌患者外周血T淋巴细胞活化相关膜分子CD3 PE和CD2 5 HLA DR CD69 FITC表达进行荧光免疫检测 ,并与正常对照组 (n =2 5)进行对比研究。结果 :50例肺癌患者外周血T淋巴细胞中CD3+ CD2 5+ 、CD3+ HLA DR+ 和CD3+ CD69+ 表达显著低于正常对照组 (P <0 .0 1 )。手术后外周血CD3+ CD2 5+ 、CD3+ HLA DR+ 和CD3+ CD69+ 表达高于手术前 (P <0 .0 1或P <0 .0 5) ,化疗前后差异无显著性 ;肺癌伴淋巴结转移CD3+ CD2 5+ 、CD3+ HLA DR+ 和CD3+ CD69+ 低于不伴淋巴结转移者 (P <0 .0 1 ) ;Ⅲ期、Ⅳ期和Ⅰ期、Ⅱ期之间CD2 5、HLA DR和CD69表达比较 ,差异有显著性 (P <0 .0 1 ) ;CD3+ CD2 5+ 、CD3+ HLA DR+ 和CD3+ CD69+ 表达与肺癌组织学分级及年龄有明显相关性 (P <0 .0 5或P <0 .0 1 ) ;与鳞癌和腺癌及患者的性别没有相关性。结论 :应用流式细胞仪检测外周血CD3+ CD2 5+ 、CD3+ HLA DR+ 和CD3+ CD69+ 的表达水平对了解患者的免疫状态、病情判断、预后评估及进行免疫治疗具有重要的意义
【Abstract】 Purpose:To study the expression of T lymphocyte activation associated membrane molecule in peripheral blood of patients with non small cell lung cancer and study the changes in its immunity function.Methods:The lymphocyte from the peripheral blood in patients with non small cell lung cancer were immunologically double fluorescent labeled by CD3 PE and CD25/ HLA DR/ CD69 FITC were determined by flow cytometry. 25 normal donors served as controls.Results:Peripheral blood CD3 + /CD25 + ,CD3 + /HLA DR + and CD3 + /CD69 + in T lymphocyte with lung cancer were significantly lower than those in normal controls ( P <0.01). CD3 + /CD25 + ,CD3 + /HLA DR + and CD3 + /CD69 + in post operative T lymphocytes were significantly higher than pre operatively( P <0.01 or P <0.05),there was no significant differences between pre chemotherapy and post chemotherapy. Lung cancer with lymph node metastasis showed lower expression of CD3 + /CD25 + ,CD3 + /HLA DR + and CD3 + /CD69 + than lung cancer without lymph node metastasis ( P <0.01). Distinct statistic differences were observed between CD3 + /CD25 + ,CD3 + /HLA DR + and CD3 + /CD69 + in stage Ⅲ,Ⅳand stageⅠ,Ⅱ( P <0.01). Histopathological grade and age were also significantly associated with the expression of CD3 + /CD25 + ,CD3 + /HLA DR + and CD3 + /CD69 + ( P <0.01 or P <0.05). Peripheral blood CD3 + /CD25 + ,CD3 + /HLA DR + and CD3 + /CD69 + contents were not related with squamous carcinoma, adenocarcinoma and gender.Conclusions:Detection of CD3 + /CD25 + ,CD3 + /HLA DR + and CD3 + /CD69 + levels by flow cytometry might be helpful for reflecting the human immunity function, judgement of disease status, prognosis evaluation and immunological treatment in patients with non small cell lung cancer.
【Key words】 non small cell lung cancer; T lymphocyte activation associated membrane molecule; flow cytometry;
- 【文献出处】 中国癌症杂志 ,China Oncology , 编辑部邮箱 ,2003年01期
- 【分类号】R734.2
- 【被引频次】3
- 【下载频次】65