【摘要】 目的　研究人脑梗死后星形胶质细胞活性及凋亡的关系。方法　应用 10例人脑梗死后的尸检全脑标本 ,常规 HE和免疫组织化学方法检测胶质纤维酸性蛋白 (GFAP)、凋亡相关蛋白 (Bcl-2 ) ,进行定性和定量分析。结果人脑梗死后以梗死灶为中心 ,按照组织损伤程度由内到外分为 4个区 (0～ 3区 ) ,其中 2区为半暗带区 ,GFAP在 0区和 1区表达很少 ,在 2区和 3区随缺血时间延长表达迅速持续升高 ;Bcl-2在 0区和 1区表达很少 ,在 2区和3区表达显著。结论　人脑梗死后在半暗带区 2 4h前 GFAP和 Bcl-2表达呈正相关 ;2 4h后呈负相关更多还原

【Abstract】 Objective To investigate the relationship between activit y of astrocytes and apoptosis in human infarcted brains.Methods W e used glial fibrillary acidic protein (GFAP) and anti-apoptotic protein (Bcl- 2) as markers to define changes of cells in post-mortem specimens from ten pati ents by HE and immunohistochemistry.Results Each section of the infarcted brain was divided into four areas (the area 0～3),and the area 2 was known as the ischemic penumbra.The expression of both GFAP and Bcl-2 was lost i n the area 0 and 1,but preserved in the area 2 and 3.GFAP selectively increased in the area 2 and 3 in a time-dependent manner.Conclusion Ther e is relationship between activity of astrocytes and apoptosis that occurred aft er ischemia.更多还原