【摘要】 目的　探讨匹罗卡品诱导慢性癫痫大鼠海马齿状颗粒细胞苔藓纤维突触重建与神经营养素受体Trk B表达的关系。方法　取匹罗卡品诱导大鼠急性癫痫持续状态及慢性自发性颞叶癫痫发作期大鼠脑片 ,用免疫组织化学方法检测 Trk B及突触体素 (P38,一种突触形成标志物 )在大鼠海马的表达。结果　急性癫痫持续状态诱导颗粒细胞表达 Trk B一过性增高 ,第 2次表达高峰呈现在 7～ 30 d;P38免疫反应性在齿状回内分子层则呈进行性增加 ,与 neo- Timm显示的异常苔藓纤维出芽相一致。结论　 Trk B受体激活有助于海马齿状回苔藓纤维轴突生长及突触形成从而有利于颞叶癫痫的发生。更多还原

【Abstract】 Objective To explore the correlation between brain derived neurotrophic factor receptor TrkB expression and dentate granule cell mossy fiber synaptic reorganization during the chronic phase following status epilepticus (SE) induced by pilocarpine in adult rats. Methods The pilocarpine (PILO) model of epilepsy was characterized by an acute period of status epilepticus followed by spontaneous recurrent seizures temporal lobe epilepsy.The time course of TrkB and Synaptophysin(P38,a marker of synaptogenesis)immunoreactivity were studied in the hippocampus during these phases in this model.Results Acute status epilepticus induced a transient increased expression of TrkB in granule cells and a second peak of TrkB expression during 7～30days,an chronic period of spontaneous recurrent seizures.Expression of synaptophysin increased progressively in the molecular layer of the dentate gyrus from silent to chronic phase,which was in line with the neo Timm stain results that revealed a time dependent growth of aberrant mossy fibers into the dentate inner molecular layer.Conclusion The results suggest that activation of TrkB receptors could contribute to the process of mossy fiber axonal outgrowth and synaptogenesis in the hippocampal dentate gyrus.This may contribute to epileptogenesis of temporal lobe epilepsy.更多还原