【摘要】 目的 了解乳腺癌细胞系NFκB家族蛋白的表达及其与化疗耐药的关系。方法 用免疫印记法分别检测了14个乳腺癌细胞系的P65,P50及IκB-α的表达,同时对10个细胞系进行MTT药物敏感实验。结果 IκB-α主要在胞浆内表达,虽然P65和P50在胞浆、胞核均有表达,但无论在胞浆还是在胞核,P50的表达均占明显优势,其在胞核的表达高达63％。P50胞核表达阳性者IC50值明显高于胞核阴性者,差异有显著意义。结论 1)乳腺癌细胞核P50表达可能预示化疗耐药,故可作为判断预后的一个新指标;2)NFκB家族蛋白中,P50的表达在乳腺癌 中更常见,且在化疗耐药性的产生中可能起着更重要的作用。更多还原

【Abstract】 Objective: To study the expression of NFκB family protein in breast cancer cell lines and the relationship between NFκB family protein and the drug resistance. Methods: Expression of P65, IκB-α in 14 breast cancer cell lines and P50 in 11 breast cancer cell lines was detected by Western blot. The sensitivity of the cells to ADM was determined by MTT. Results: IκB-α located mainly in the cytoplasm. P65 and P50 were in both of cytoplasm and nucleus. The expression level of P50 was higher than that of P65, especially in nucleus. MTT assay showed that IC50 was three-fold higher in the cell lines which expressed P50 in nucleus than those P50 negative in nucleus, but no difference was found in the expression of P65. Conclusion: The expression of P50 in nucleus may predict the chemotherapy resistance in breast cancer, so it can be used as an indicator to predict the prognosis. The expression of P50 is more often in breast cancer, and it may play a more important role in the chemotherapy resistance than other NFκB family members.更多还原