【摘要】 目的　研究不同生长调节物质和有机添加物对霍山石斛原球茎试管再生苗生根的影响。方法　将试管苗分别培养在添加不同浓度的 NAA,IBA,BA,KT,香蕉泥或氨基酸的 MS基本培养基中诱导生根 ,观察并统计试管苗生根状况。结果　不同 NAA,IBA,BA ,KT,香蕉泥或氨基酸添加浓度对试管苗生根率、根长、生根数及长势的影响有明显差异 ,它们的适合浓度分别为 0 .2 ,0 .1,0 .8,0 .8m g/ L,2 0 %和 0 .1%。结论　 NAA,IBA和香蕉泥对霍山石斛原球茎试管再生苗生根影响较大 ;氨基酸 ,BA和 KT对生根作用不明显 ;KT有持绿作用。更多还原

【Abstract】 Object To study the effects of different growth regulators and organic additives on rooting of regenerated shoots from Dendrobium huoshanense C Z Tang et S J Cheng protocorm like bodies Methods In order to induce root development, the regenerated shoots were cultured on MS medium containing 3% sucrose and 0 8% agar and supplemented with NAA, IBA, BA, KT, banana mud or amino acids at different concentrations During rooting induction, the development of roots was observed and the frequency of rooted shoots was scored Results The frequency and the process of root initiation were significantly influenced by different growth regulators or organic additives For initiation and development of roots, the suitable concentration of NAA, IBA, BA, KT, banana mud or amino acids was 0 2 , 0 1, 0 8, 0 8 mg/L, 20% and 0 1%, respectively Conclusion In all media tested, NAA, IBA or banana mud display greater effects on rooting of regenerated shoots of D huoshanense than others and KT enables shoots to keep green更多还原