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黄褐毛忍冬不同炮制品中绿原酸的HPLC法含量测定

Determination of chlorogenic acid in different processed crude drugs of Lonicera fulvotomentosa by HPLC

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【作者】 张艳焱; 张天伦; 梁光义; 曹佩雪; 阮婧华;

【Author】 ZHANG Yan yan 1, ZHANG Tian lun 1, LIANG Guang yi 2, CAO Pei xue 2, RUAN Jing hua 2 (1. Guiyang College of TCM, Guiyang 550002, China; 2. The Key Laboratory of Chemistry for Natural Products of Guizhou Province and Chinese Academy of Sciences, Guiyang 550002, China)

【机构】 贵阳中医学院; 贵州省、中国科学院天然产物化学重点实验室; 贵州省、中国科学院天然产物化学重点实验室 贵州贵阳550002; 贵州贵阳550002; 贵州贵阳550002;

【摘要】 目的 比较黄褐毛忍冬花蕾、白花、黄花生晒品及蒸晒品中绿原酸的含量。方法 采用 HPLC法 ,反相 Hy-persil C1 8柱 ,以乙腈 -0 .4%磷酸溶液 (13∶ 87)为流动相 ,检测波长为 3 2 7nm。结果 黄褐毛忍冬花蕾、白花、黄花生晒品绿原酸含量分别为 0 .5 4% ,0 .81% ,0 .5 7% ;蒸晒品中绿原酸含量分别为 2 .14 % ,1.65 % ,2 .0 4%。结论 蒸晒法较生晒法炮制的黄褐毛忍冬中绿原酸含量高

【Abstract】 Object To compare the contents of chlorogenic acid in the different processed crude drugs of Lonicera fulvotomentosa Hsu et S.C. Cheng. Methods The contents of chlorogenic acid were deter mined by HPLC. Chromatographic conditions included Hypersil C 18 column and the mobile phase consisting of a mixture of acetonitrile 0 4% phosphoric acid (13∶87). Chlorogenic acid was detected at 327 nm wavelength. Results The contents of chlorogenic acid were 0 54%, 0 81%, 0 57% in the sun cured flower bud, white bloom, gold flower in L. fulvotomentosa and 2 14%, 1 65%, 2 04% in the steaming sun cured crude drugs, respectively. Conclusion The contents of chlorogenic acid in the steaming sun cured crude drugs are higher than those in the sun cured crude drugs.

【基金】 贵州省重大攻关项目资助 [黔科社农字 ( 2 0 0 0 ) 114 2号 ]
  • 【文献出处】 中草药 ,Chinese Traditional and Herbal Drugs , 编辑部邮箱 ,2003年08期
  • 【分类号】R284.1
  • 【被引频次】19
  • 【下载频次】224
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