【摘要】 目的　应用细胞因子寡核苷酸表达谱芯片检测血虚小鼠不同脏器组织内造血相关细胞因子差异表达情况 ,寻找差异表达基因 ,为血虚证治疗药物机制研究及治疗药物筛选奠定基础。方法　采用 5 .5 Gy6 0 Co- γ射线照射Balb/c小鼠 ,制备血虚模型。在不同时间点提取正常和血虚小鼠不同组织总 RNA,反转录成不同荧光标记的c DNA探针 ,与表达谱芯片进行杂交 ,对扫描数据进行分析获得血虚小鼠造血相关细胞因子基因的差异表达情况。结果　在各组织中共发现 2 1个差异表达的基因。这些基因的功能大致分为 3类 :促细胞生长或增殖 ,免疫调节 ,诱导血细胞形成或促造血祖细胞形成集落。结论　照射后上述细胞因子基因的下调 ,使机体的造血功能下降 ,造成血虚 ,证明细胞因子间形成造血调节网络 ,整体调节机体造血。这与中医的全局理论是一致的。实验证明应用芯片技术 ,从分子水平研究血虚形成的机制是可行的 ,可为进一步的血虚证治疗药物的筛选提供依据更多还原

【Abstract】 Object To study the hematopoietic related genes differential expression in blood deficiency mice tissues using oligonucleotide microarray in order to lay a fundation of investigating on the treatment mechanism of blood deficiency and screening the treatment drugs. Methods Balb/c mice were irradiated with 5.5 Gy 60 Co γ to make model of blood deficiency in mice. The total RNAs were isolated from different tissues with Trizol reagent by one step method at different time, then reverse transcribed into cDNA probe with the incorporation of fluorescent dUTP. The mixed probe was hybridized to the self made microarrays, then scanned and analyzed with Bio information software. Results There were 21 hematopoietic related genes expressed decreasingly in all the detected tissues except spleen irradiated with 60 Co γ. The functions of all differential expression genes were classified into three classes: prompting cell growth or proliferation, regulating immunization, inducing the coloning of the hematopoietic progenitor cells. Conclusion The hematopoietic related genes which changed in blood deficiency mice play important roles in a network to regulate the hematopietic system. The network regulation agree with the “whole theory” in Chinese medical science. The experiments have confirmed that it is possible to explore the hematopoietic mechanism at molecular level with the microarrays, and the further study can be carried out in this field.更多还原