【摘要】 目的　建立臭冷杉挥发油中有效成分柠檬烯的毛细管气相色谱测定方法。方法　色谱条件为 SE- 5 4毛细管色谱柱 ,程序升温 ,分流比为 4 0∶ 1,FID检测器 ,内标为萘。结果　柠檬烯浓度在 0 .6 0 132～ 1.4 0 30 8mg/ m L 呈线性关系 (r=0 .9996 ) ,平均加样回收率为 99.6 2 % (RSD=2 .4 1% )。结论　该法简便 ,灵敏 ,易操作 ,可用于臭冷杉生药的质量评价更多还原

【Abstract】 Object To develop a reliable method for determination of limonene in volatile oil of Abies nephrolepis (Trautv.) Maxim. leaves by capillary gas chromatography (CGC). Methods SE 54 quartz capillary column was used and the temperature of column was programmed. The splitting ratio was 40∶1 The FID was used as detector, and the naphthalene as internal standard. Results The standard curve was linear in the concentraion range of 0 601 32 — 1 403 08 mg/mL (r=0 999 6). The average recovery was 99 62% with RSD=2 41%. Conclusion The method can be used for quality assessment of A. nephrolepis leaves and it is rapid, sensitive, precise and has good reproducibility.更多还原