【摘要】 目的 :研究抗纤灵颗粒剂 (丹参、制大黄、牛膝等 )对慢性肾衰竭肾纤维化、肾功能的作用。方法 :与蒙诺相对照 ,观察抗纤灵颗粒剂对慢性肾衰竭患者及 5 /6肾切除制作的慢性肾衰实验大鼠的肾功能指标即血清肌酐 (Scr)、尿素氮(BUN)和纤维化指标即血浆层粘连蛋白 (LN)、纤维联结蛋白 (FN)、Ⅲ型前胶原 (PC Ⅲ )、Ⅳ型胶原 (C Ⅳ )的影响 ,并应用免疫组化技术观察抗纤灵颗粒剂及其拆方对实验大鼠残余肾组织细胞外基质FN、C Ⅳ的作用。结果 :抗纤灵颗粒剂有明显的降低慢性肾衰竭患者及实验大鼠的血清Scr、BUN、LN、PC Ⅲ、C Ⅳ和升高FN的作用 ,并有降低CRF大鼠肾小球硬化指数和肾组织FN、C Ⅳ的表达 ,具有统计学意义。结论 :抗纤灵颗粒剂能减轻肾纤维化、延缓慢性肾衰竭的进程更多还原

【Abstract】 Objective: To observe the effect of Kangqianling Granules (Radix Salviae miltiorrhizae,Radix et Rhizoma Rhei, Radix Achyranthis Bidentatae, etc.) on renal function and renal fibrosis in chronic renal faiure(CRF). Methods: Effect of Kangqianling Granules on Serum Creatinine (SCR), Blood Urea Nitrogen (BUN), Laminin (LN), Fibronectin (FN), Precollagen Ⅲ (PC Ⅲ) and Collagen Ⅳ (C Ⅳ) were observed in CRF patients and rats with 5/6 nephrectomy. The expression of FN and C Ⅳ in remnent renal tissue of model rats were determined by immunhistochemistry. Results: Kangqianling Granules decreased significently serum volume for Scr, BUN, LN, PC Ⅲ, C Ⅳ and elevated FN considerably in CRF patients and rats. Kangqianling Granules inhibited the expressions of FN and C Ⅳ protein in remnant renal tissue of model rats. Conclusion:Kangqianling Granules could relieve renal fibrosis and delay process of CRF.更多还原