【摘要】 目的 :研究大孔吸附树脂富集、纯化茅霉总皂苷的工艺条件及参数。方法 :以茅莓总皂苷的洗脱率和纯度为考察指标 ,研究大孔吸附树脂富集、纯化茅莓总皂苷的吸附性能和洗脱参数。结果 :茅莓提取液 1 9.3mL(0 .77mg·mL- 1 )上大孔吸附树脂柱 (干重 2 .68g ,1cm× 2 0cm) ,用蒸馏水 1 0 0mL、50 %乙醇 1 0 0mL依次洗脱 ,茅莓总皂苷富集于 50 %乙醇洗脱液部分 ,且除杂质效果好。结论 :通过大孔吸附树脂富集、纯化后 ,茅莓总皂苷的洗脱率在 79.2 %以上 ,总固物中茅莓总皂苷含量可达 55 .3 %。说明采用本法富集、纯化茅莓总皂苷是可行的更多还原

【Abstract】 Objective: To study the technological parameters of the purification process of total saponins of Rubus parvifolius L. with macroreticular resin. Methods: The adsorptive characteristics and elutive parameters of the process were studied by taking the elutive and purified ratio of total saponins of Rubus parvifolius L. as marker. Results: 19.3mL of the extractive of total saponins of Rubus parvifolius L. was purified with a column of macroreticular resin (R1cm×H20cm,dried weight 2.68g) and was washed with 100mL of distilled water, then was eluted with 100mL of 50% ethanol. Conclusion: With macroreticular resin to adsorb and purify, the elutive ratio of total saponins of Rubus parvifolius L. was over 79.2% and the purity reached 55.3%. So this process of applying macroreticular resin to adsorb and purify total saponins of Rubus parvifolius L. is feasible.更多还原