【摘要】 目的 :研究重组人白细胞介素 - 13(rhIL - 13)对巨核细胞系 -Dami细胞原癌基因c -mpl表达的影响。方法 :用RT -PCR法检测c-mplmRNA的表达。用配体结合实验检测膜蛋白c-mpl的表达。结果 :经 2 5 μg/LrhIL - 13处理过的实验组Dami细胞c -mplmRNA的表达比对照组高 2 4 8%。经 10 0 μg/LrhIL - 13处理过的实验组Dami细胞膜蛋白c -mpl的表达比对照组高 2 8 5 % (P <0 0 5 )。结论 :rhIL - 13增强了Dami细胞原癌基因c -mpl表达 ;膜蛋白c-mpl与促血小板生成素 (TPO)的结合功能正常 ;rhIL - 13促进Dami细胞增殖的部分机制可能是通过上调原癌基因c -mpl的表达来实现的更多还原

【Abstract】 AIM: To investigate the effects of recombinant human interleukin-13 (rhIL-13) on the expression of proto-oncogene c-mpl in Dami cells, a human megakaryobiastic leukemia cell line. METHODS: The expression of c-mpl mRNA in Dami cells was investigated with RT-PCR. The expression of membrane-bound protein c-mpl on Dami cells was investigated with ligand combination experiment. RESULTS: In RT-PCR experiment, we found the quantitis of expression of c-mpl mRNA in 25 μg/L rhIL-13 group increased by 24.8%. In ligand combination experiment, we found quantitis of expression of membrane-bound protein c-mpl in 100 μg/L rhIL-13 group increased by 28.5% ( P<0.05 ). CONCLUSION: Our data indicate that rhIL-13 enhances the expression of proto-oncogene c-mpl in Dami cells. Membrane-bound protein c-mpl on the surface of Dami cells bind to TPO (thrombopoietin) normally, moreover, the proliferation of Dami cells stimulated by rhIL-13 may be partly due to increasing the proto-oncogene c-mpl expression in Dami cells.更多还原