【摘要】 目的 :探讨人巨细胞病毒 (HCMV)是否通过激活核转录因子 (NF -κB)调控宿主细胞增殖与凋亡。方法 :用HCMVAD16 9株感染人胚肺成纤维细胞 (HEL) ,采用免疫组化和Westernblot方法检测宿主细胞NF -κB和Bcl- 2蛋白的表达与I-κBα的变化 ,应用MTT方法观察细胞的增殖活性 ,同时用流式细胞术检测细胞凋亡指数。结果 :HCMV感染后 4 8h细胞核表达NF -κB蛋白最多 ,2 4hI-κBα降到最低 ,12 0hNF -κB失活 ,bcl- 2的表达与NF-κB的活性改变一致。HCMV感染在 72h前抑制宿主细胞凋亡 ,促进细胞增殖 ,在 12 0h可诱导宿主细胞凋亡。结论 :NF -κB在HCMV感染诱导宿主细胞增殖与凋亡中起作用 ,与HCMV疾病的发生发展有关更多还原

【Abstract】 AIM: To investigate whether human cytomegalovirus(HCMV) regulate human embryonic lung fibroblast(HEL) cell proliferation and apoptosis by activating NF-κB. METHODS: Immunohistochemistry and Western blot analysis were used to detect the NF-κB translocation and/or Bcl-2 and the levels of I-κBα during HCMV infection. Apoptotic cell were examined by flow cytometry, and the HEL cell proliferation was determined by MTT. RESULTS: The levels of NF-κB in the nucleus reached highly 48 h postinfection, and the levels of I-κBα were low 24 h postinfection. The activity of NF-κB was inhibited 120 h postinfection. The levels of bcl-2 was accorded with the activity of NF-κB. HCMV promoted HEL cells to proliferate before 72 h postinfection and induced apoptosis 120 h postinfection. CONCLUSION: NF-κB plays a role in HEL cell proliferation and apoptosis during HCMV infection, and it involves in the pathological mechanisms of diseases associated with HCMV infection.更多还原