【摘要】 目的 :研究肾性高血压大鼠发病与心脏α1 肾上腺素能受体自身抗体的关系。方法 :利用手术的方法建立肾性高血压大鼠模型 ,观察心脏α1 肾上腺素能受体自身抗体与肾性高血压发病的关系 ,同时对自身抗体生物活性进行分析。结果 :在狭窄左肾动脉两周后 ,大鼠血浆中心脏α1 肾上腺素能受体自身抗体的阳性率和滴度与术前相比均明显升高 ,并且可持续到 1 2周 ,以后逐渐下降 ;同时心脏α1 肾上腺素能受体自身抗体对培养的乳鼠心肌细胞的跳动具有激动剂样效应。结论 :心脏α1 肾上腺素能受体自身抗体可能通过作用使外周血管阻力增加 ,从而促进肾性高血压大鼠心肌肥厚更多还原

【Abstract】 AIM: To examine the autoantibody against α 1-adrenoceptor and its biologic activities during the development of renal hypertension. METHODS: Renal hypertension of rat was achieved by clipped renal artery, the titre of autoantibody to α 1-adrenoceptor was detected using ELISA immunoassay. Furthermore, the biological offects of these autoantibodies on cultured cardiomyocytes were also examined. RESULTS: After two weeks of clipping renal arteries, both the frequency of occurrence and the titre of autoantibodies to cardiac α 1-adrenergic receptor were significantly increased as compared with the control of pre-treatment. The increased autoantibodies lasted for several weeks and then automatically decreased gradually to the pre-clipping level at 12 weeks. The biological effects of these autoantibodies displayed an "agonistic-like" activities on the beating frequency of cultured neonatal cardiomyocytes. CONCLUSION: Autoantibodies against α 1-adrenoceptor may play a role in the elevation of peripheral vascular resistance and in the development of cardiac hypertrophy in rats with renal hypertension.更多还原