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应用基因表达谱芯片观察小鼠心肌缺血后基因表达的变化以及四逆汤对其影响

Study on differentially expressed genes in ischemic myocardium and the effect of Sini decoction on them by DNA microarray

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【作者】 吴伟康谭红梅罗汉川赵明奇梁天文

【Author】 WU Wei-kang, TAN Hong-mei, LUO Han-chuan, ZHAO Ming-qi, LIANG Tian-wen (Departmetn of Pathophysiology, Sun Yat-sen University of Medical Sciences, Guangzhou 510089, China)

【机构】 中山大学中山医学院病理生理学教研室中山大学中山医学院病理生理学教研室 广东广州510089广东广州510089广东广州510089

【摘要】 目的 :应用基因表达谱芯片观察小鼠心肌缺血后基因表达的变化以及四逆汤对其影响。方法 :昆明种小鼠 ,随机分为对照组、缺血组、缺血加四逆汤组。提取各组心肌组织总RNA ,纯化mRNA ,与含有 2 30 4条小鼠基因的cDNA表达谱芯片进行杂交。结果 :小鼠缺血后有 33条基因表达下调 ,70条基因表达上调 ;服用四逆汤后 ,相对单纯缺血组而言 ,有 2 3条基因表达下调 ,52条基因表达上调。结论 :运用基因芯片技术能快速地检测出缺血心肌及四逆汤治疗后心肌基因表达谱的改变 ,对差异表达基因的研究将有助于进一步了解心肌缺血及四逆汤治疗的分子机制。

【Abstract】 AIM: To screen the differentially expressed genes among normal, ischemic and Sini decoction-treated myocardium using DNA microarray.METHODS: Kunming mice were randomly divided into control group, ischemic group and Sini decoction group. Total RNA was extracted from myocardium of each group. cDNA microarray chips containing 2 304 cDNAs were used to investigate the gene expression pattern of each group. RESULTS: Up-and down-regulated genes were 33 and 70 in ischemic group vs control group, respectively. Up-and down-regulated genes were 23 and 52 respectively in Sini decoction group vs ischemic group. CONCLUSIONS: The analysis of gene expression pattern of ischemic myocardium based on cDNA microarray can realize high-throughput screening of the genes. Further analysis of those obtained genes information will be helpful to understand the molecular mechanism of myocardial ischemia and the therapeutic mechanism of Sini decoction.

【基金】 国家自然科学基金资助项目 (No .39770 936);广东省自然科学基金资助项目 (No .960 1 1 3)
  • 【文献出处】 中国病理生理杂志 ,Chinese Journal of Pathophysiology , 编辑部邮箱 ,2003年02期
  • 【分类号】R363
  • 【被引频次】27
  • 【下载频次】274
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