【摘要】 目的 :探讨TF - 1细胞凋亡相关基因 1 9(TFAR1 9)在系统性红斑狼疮 (SLE)发病诱因中的作用及其与SLE病情的关系。方法 :应用倒置相差显微镜、Ladder方法、Westernblotting方法、荧光免疫显微镜、ELISA方法检测中波紫外线 (UVB)诱导HaCaT细胞凋亡时TFAR1 9的表达及其与SLE患者病情的关系。结果 :在剂量为 30mJ/cm2的UVB照射 2 4h后 ,HaCaT细胞开始凋亡 ,TFAR1 9在此过程中有表达 ,发现活动期SLE患者血清中TFAR1 9的抗体值高于稳定期和正常对照组的值 (P≤ 0 .0 5)。结论 :在UVB诱导角质形成细胞HaCaT凋亡的过程中 ,有TFAR1 9的参与并发挥了作用 ,提示TFAR1 9可能和SLE的发病和病情有关更多还原

【Abstract】 AIM: To clarify the role of new apoptosis-related gene, TF-1 cell apoptosis-related gene 19(TFAR19), in the pathogenesis of systemic lupus erythematosis (SLE) and the relationship between TFAR19 and SLE. METHODS: DNA Ladder detection, Western blotting, immunological fluorescence method, ELISA and so on were used to test if ultraviolet B(UVB) could induce HaCaT cell apoptosis and TFAR19 expression. RESULTS: HaCaT cell apoptosis could be detected after 24 hours of 30 mj/cm 2 UVB irradiation. Also, we found that in active SLE patients, the TFAR19 antibody was increased, but not significant compared to the normal control. CONCLUSION: TFAR 19 is involved in the process of UVB induced ketatinocyte line HaCaT apoptosis and SLE pathogenesis.更多还原