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4种肾炎模型大鼠尿液中补体C5b-9复合物排泄的比较研究

A comparative study on urinary complement C5b-9 complex excretion in four nephritis models of rats

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【作者】 王迎伟简洁汤仁仙黄水平

【Author】 WANG Ying-wei 1, JIAN Jie 2, TANG Ren-xian 2,HUANG Shui-ping 3 (? 1?Department of Immunology,Nanjing Medical University, Nanjing 210029, China; ? 2?Department of Immunology,? 3?Department of Statistics, Xuzhou Medical College, Xuzhou 221002, China)

【机构】 南京医科大学免疫学教研室江苏徐州医学院免疫学教研室江苏徐州医学院统计学教研室 江苏南京210029现单位:南京医科大学微生物学与免疫学教研室江苏南京210029江苏徐州221002江苏徐州221002

【摘要】 目的 :探讨不同类型的免疫复合物肾炎大鼠尿液中补体C5b - 9复合物检出的意义。方法 :复制大鼠被动型Heymann肾炎 (PHN)、抗胸腺细胞血清性肾炎 (ATSN)、抗肾小球基底膜性肾炎(AGBMN)及慢性血清病肾炎 (CSDN) 4种动物模型。用双抗体夹心ELISA法定期检测各种肾炎大鼠血液及尿液中补体C5b - 9的含量 ,同时观察大鼠肾小球中C5b - 9的沉积情况。结果 :4种肾炎模型鼠血浆C5b - 9复合物均见上升 ,肾小球内也有C5b - 9复合物的沉积 ,但尿液中C5b - 9含量升高仅见于罹患PHN的大鼠。此外 ,尿液中C5b - 9复合物的升高先于PHN大鼠尿蛋白的升高。结论 :尿液中C5b - 9复合物的检测可作为PHN早期诊断和鉴别诊断的一种敏感性较高的免疫学指标

【Abstract】 AIM: To explore the significance of measuring urinary complement C5b-9 complex in various types of immune complex (IC) nephritis models of rats. METHODS: The four models of rats, namely, passive Heymann nephritis (PHN), anti-thymocyte serum nephritis(ATSN), anti-glomerular basement membrane nephritis (AGBMN) and chronic serum disease nephritis (CSDN) were reproduced. Then, the contents of complement C5b-9 complex in plasma and urine of the rats were detected with sandwich ELISA. And the deposits of C5b-9 complex in glomeruli of the rats were examined by ABC immunohistochemistry staining. RESULTS: The contents of rat plasma C5b-9 were elevated and deposits of C5b-9 in glomeruli could be detected in the four model rats. But the increased urinary excretion of C5b-9 was observed only in PHN rats. Moreover, the time of urinary C5b-9 complex excretion was earlier than that of urinary protein in the rats with PHN. CONCLUSION: Urinary C5b-9 complex excretion could be taken as one of several sensitive immunologic parameters in diagnosing of PHN and in distinguishing PHN from other type of nephritis.

【关键词】 肾炎补体C5b-9复合物大鼠
【Key words】 NephritisComplement C5b-9 complexRats
【基金】 江苏省教委基金资助项目 (No .960 95);江苏省自然科学基金资助课题(No.BK971 54);江苏省“333”工程基金资助项目 (No .2 0 0 78)
  • 【文献出处】 中国病理生理杂志 ,Chinese Journal of Pathophysiology , 编辑部邮箱 ,2003年01期
  • 【分类号】R692.3
  • 【被引频次】5
  • 【下载频次】235
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