【摘要】 目的 :观察急性缺氧时血浆、组织内源性一氧化碳的变化及外源性一氧化碳对急性肺动脉高压的影响。方法 :实验分为两部分 :实验 1 :30只雄性Wistar大鼠随机分为 3组 :组I (对照组 ) :大鼠吸入 2 1 %O23 0min ;组II(缺氧组 ) :大鼠吸入 1 0 %O2 3 0min ;组III (ZnPPIX组 ) :腹腔注入血红素氧化酶抑制剂ZnPPIX 1 0μmol/kg后 ,大鼠吸入 1 0 %O2 3 0min。实验结束时取血样进行一氧化碳及环磷酸鸟苷分析 ,取组织样品进行匀浆、离心测组织一氧化碳含量 ;实验 2 :1 0只大鼠吸入 1 0 %O2 2 0min ,待肺动脉压力曲线恢复基线后 ,给大鼠吸入50× 1 0 - 6 的一氧化碳与缺氧气体的混合气 30min ,观察血流动力学和血气的变化。结果 :急性缺氧增加了血浆、组织一氧化碳含量及血浆环磷酸鸟苷含量 ,ZnPPIX降低了缺氧大鼠的血浆、组织一氧化碳含量及血浆环磷酸鸟苷含量 ,缺氧明显升高了平均肺动脉压 ,而吸入 50× 1 0 - 6 的一氧化碳可明显降低肺动脉压 ,而且吸入一氧化碳可明显增加缺氧大鼠的动脉氧分压。结论 :急性缺氧明显增加了血浆、组织一氧化碳含量及血浆环磷酸鸟苷含量。外源性一氧化碳可明显降低缺氧时肺动脉高压更多还原

【Abstract】 AIM: To investigate changes of carbon monoxide (CO) in plasma and tissue during acute hypoxia and effect of exogenous CO on acute hypoxic pulmonary arterial hypertension. METHODS: Experiment was divided into two protocols : Protocol 1: 30 Male Wistar rats (180-250 g) were randomly assigned into three groups: Group I (control) : rats were inhaled with 21%O 2 for 30 min. Group II (hypoxia) : rats were inhaled with 10%O 2 for 30 min. Group III (ZnPPIX): after heme oxygenase (HO) inhibitor ZnPPIX was administered, rats were inhaled with 10%O 2 for 30 min. At the end of experiment, blood samples were collected for carbon monoxide and cGMP assay. Tissue samples were homogenized and centrifuged for CO assay. Protocol 2: 10 male Wistar rats (180-250g) were inhaled with 10%O 2 for 20min. When the pressure curve of mean pulmonary arterial pressure returned to baseline, the mixture of 50×10 -6 CO and hypoxic gases was administered to rats and the change of mean pulmonary arterial pressure (MPAP) was also observed. RESULTS: Hypoxia increased CO levels in plasma and tissue as well as cGMP level in plasma. ZnPPIX reduced CO levels of plasma and tissue as well as cGMP level of plasma in hypoxic rats. Hypoxia elevated MPAP significantly while inhalation of 50×10 -6 CO reduced MPAP markedly. Moreover, CO increased PaO 2 significantly in hypoxic rats. CONCLUSION: Acute hypoxia increased CO levels in plasma and tissue as well as cGMP levels in plasma. Exogenous CO reduced MPAP significantly.更多还原