To construct the recombinant plasmid containing envelope glycoprotein E2 gene of RV JR23 strain, to express E2 protein in BHK21 cells, and to analyze the antigenic sites of E2 protein of RV JR23 strain, E2 gene of RV JR23 strain was amplified by RT-PCR. The PCR product was cloned into the expression vector pBluescript Ⅱ SK~+ and transformed into E. coli JM109, and the recombinant plasmid was transfected into BHK21 cells with recombinant vaccinia virus VTF-7. The expression was detected by immunohistochemist...
| 
推荐 
点击下列相关研究机构和相关文献作者，可以直接查到这些机构和作者被《中国知识资源总库》收录的其它文献，使您全面了解该机构和该作者的研究动态和历史。
