【摘要】 采用NH₄C1弱碱法处理感染口蹄疫病毒的叙利亚仓鼠肾细胞系（BHK-21）,存活细胞经过单克隆和选择,获得32株阳性克隆细胞株。随机选取一株（BHK-ROp）,常规传代并采用RT-PCR,透射电子显微镜,流式细胞仪进行持续感染特性分析,结果表明,BHK-ROp第4,16,36代细胞中病毒持续存在,但不影响细胞的生长特性,表现出病毒持续感染的基本特征。可见,NH₄C1弱碱法用于建立病毒持续感染细胞系是十分有效的。更多还原

【Abstract】 A baby hamster kidney cell line（BHK-21） infected by Foot-and-mouth disease virus （OGBF15 strain） was treated with NH₄Cl, then the cells survived were selected and cloned. 32 positive-cloned cell strains were obtained. One strain （BHK-ROp） was randomly selected and subcultured as usual and analysed with RT-PCR, transmission electron microscope and flow cytometry. The results demonstrated that the 4th, 16th, 36th generation BHK-ROp cell still harboured FMDV which had no changes on the growth features of the cells. They had the radical characteristics of viral persistent infection. As noted above, the NH₄Cl weak base method used for establishment of the cell line persistently infected with virus was particularly effective.更多还原